摘要
目的 用重离子12 C照射离体人血建立染色体畸变的剂量 -效应曲线。方法 重离子12 C照射离体人血 ,吸收剂量为 1 0～ 8 0Gy ,剂量率为 3 0Gy/min。染色体采用微量法 ,培养开始加秋水仙硷 ,主要记录染色体型畸变的非稳定性畸变 ,对实验数据做曲线拟合 ,并检验回归系的显著性和曲线的拟合度。结果 重离子12 C照射离体血诱发的染色体畸变 (双 +环 ) ,在 0～ 8 0Gy范围内 ,呈良好的剂量 -效应关系 ,拟合的最佳回归方程为Y=0 85 85 0 3D +0 3 615 × 10 -2 D2 。结论 重离子12 C照射离体人血在 0～ 8 0Gy范围内 ,“双 +环”拟合的最佳数学模式为Y =bD +cD2 。
Objective To established the curves of relationships between radiation dose and chromosome aberrations induced by heavy ion in human peripheral blood lymphocytes in vitro.Methods Heparinized whole blood samples were exposed to heavy ion 12C.Radiations doses were from 1.0 Gy to 8.0 Gy to 8.0 Gy.Dose rate was 3.0 Gy/min.Unstable chromosome aberrations including acentric fragments,minutes,acentric rings,dicentrics and cenric rings(“dic+r”)were scored.Results Chromosome aberrations(“dic+r”)induced by heavy ion 12C had a good dose-response relationships.Fitting the best regression equation was Y=0.858503D+0.3615×10 -2D 2.Conclusion When human blood irradiated by heary ion 12C within 0～8.0 Gy,the best mathematical model of chromosome aberrations(“dic+r”) was Y=bD+cD 2.
出处
《中国辐射卫生》
2003年第2期67-69,共3页
Chinese Journal of Radiological Health
