摘要
目的 :探讨雄黄对NB4细胞 (维甲酸敏感的急性早幼粒细胞白血病细胞株 )和MR2 细胞 (维甲酸耐药的急性早幼粒细胞白血病细胞株 )促凝活性 (PCA)和组织因子 (TF)表达的影响。方法 :用 30 0 μg·L-1雄黄与NB4和MR2 细胞共同孵育 ,分别用复钙时间、ELISA方法、半定量RT PCR检测未处理及雄黄处理 6 ,12 ,2 4 ,4 8,72h后NB4和MR2 细胞的促凝活性、TF抗原表达及TFmRNA转录的变化。同时检测未处理的HL 6 0 ,K5 6 2细胞的PCA ,TF抗原表达作为对照。结果 :NB4和MR2 细胞的PCA和TF抗原水平明显高于HL 6 0和K5 6 2细胞。雄黄呈时间依赖方式下调NB4和MR2 细胞的促凝活性 ,TF抗原的表达及TFmRNA的转录。结论 :下调NB4和MR2 细胞TF的表达并降低其PCA可能是雄黄改善APL患者弥散性血管内凝血 (DIC)早期出血症状的主要机制之一。
Objective: To investigate the effects of Realgar on procoagulant activity (PCA), tissue factor expression and tissue factor mRNA transcription in acute promyelocytic leukemia (APL) cell lines NB4 and MR2 cells. Method: NB_4 and MR_2 cells were treated with 300 μg·L -1 Realgar PCA of the treated cells was detected using one-stage clotting assay. TF antigen was detected by ELISA and TFmRNA by semi-quantitive RT-PCR. Result: The PCA and TF antigen level in NB_4 and MR_2 cells were significantly higher than that in HL-60 and K562 cells. Realgar could down-regulate the membrane PCA, TF antigen and TF mRNA transcription of NB_4 and MR_2 cells in a time-dependent manner. Concluson: Down-regulating TF expression and PCA of NB_4 and MR_2 cells by Realgar may be one of the mechanism of its improvement effect on DIC-related hemorrhage of APL patients.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2003年第6期553-556,共4页
China Journal of Chinese Materia Medica
基金
陕西省中医管理局基金资助项目 (19990 0 2 )
