摘要
用石蜡切片和扫描电镜对墨兰试管苗植株的生长发育进行了研究。发现幼叶中脉附近的叶肉细胞类似栅栏组织,随着叶片的不断成熟,叶片基部中脉附近的叶肉细胞逐渐变为近圆形或椭圆形,而叶尖部和叶中部中脉附近的叶肉细胞仍似栅栏组织。茎的发育经历了原球茎、根状茎和假鳞茎3个阶段。原球茎的大部分细胞都含有淀粉粒,根状茎的皮层细胞含淀粉粒,而假鳞茎几乎不含淀粉粒。幼根没有髓,皮层细胞含淀粉粒;成熟根具含淀粉粒的髓。出瓶苗上即带有4个芽,一般只有最外侧叶腋的1个花芽和最内侧叶腋的1个叶芽发育。
The growth and development of Cymbidium sinense in vitro were observed under scanning electron microscopy.The cells in mesophyll near midrib are often elongated as palisade-liked tissue in young leaves.With the development of the plantlets,cells in mesophyll near midrib of leaf base are found toi be gradually rounded or elliptic,but those in leaf apex and near midrib are still arranged as palisade-liked tissue.The development of the stem goes through protocorm,rhizome and pseudobulb stages.There are a large number of starch grains in most cells of protocorm and in cells of cortex parenchyma of rhizome,but almost no starch grain is found in pseudobulb.In young roots,medullas are absent and starch grains scatter in cortex parenchyma.In mature roots,there are medullas with starch grains.Generally,of the four axillary buds,only the outmost floral bud and the innermost leaf bud are developmental.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2003年第2期161-165,T002,T003,共7页
Journal of Tropical and Subtropical Botany
基金
中国科学院院长基金(767)
广东省科技计划项目(C20304)
关键词
墨兰
试管苗
个体发育
解剖
Cymbidium sinense
Plantlet in vitro
Ontogeny
Anatomy
