摘要
我们观察了ELISA法测定HBsAg显色时的不同温育温度及时间变化,对测定结果有较大影响,随着温度的升高和时间的延长,P值及P/N值逐渐升高。因而在实际工作中,应选择一最适的显色温度和时间,以稳定室内质控的结果。
We observed the effect of different incubation temperatures and time in the process of color reaction on the results of HBsAg determined by ELISA. The results showed that different temperatures and times in the process of color reaction had great effect on the results. The values of P and P/N rise gradually along with increasing temperatures and time. Thus it is necessary to choose the most proper temperature and time in the process of color reaction in order to get stable internal qualty control result in pratical work.
