摘要
目的 :采用静脉注射神经生长因子偶联物 (NGF- Tf)的方法 ,研究其对痴呆 (AD)大鼠的影响。方法 :选用手术切断大鼠双侧隔——海马胆碱能通路的方法建立 AD动物模型 ,每天从大鼠尾静脉注射 NGF- Tf。分别以跳台试验和水迷路试验观察大鼠的学习记忆以及方向辨别能力 ;并对相应海马和隔区行神经组织学检查。结果 :模型组跳台试验错误次数增加 ,在训练期与正常组比较有统计学意义 (P <0 .0 5 ) ;NGF- Tf组错误次数较少 ,与模型组比较 ,在训练期差异有统计学意义 (P <0 .0 5 ) ,测验期错误次数减少率亦可达 5 0 %。水迷路试验中 ,NGF- Tf组在 1 0 s内抵达平台的正确反应平均数提高 ,与模型组比较 ,差异有统计学意义 (P <0 .0 5 )。光镜下 ,NGF- Tf组隔区仅见轻微萎缩性改变 ,而模型组隔区萎缩性改变较明显 ,表现为神经元数目明显减少 ,细胞轮廓不清 ,有胶质细胞增生。正常对照组、NGF- Tf组和模型组的海马区未见明显病理性改变。结论 :NGF-Tf能穿透血脑屏障 ,有效防止模拟 AD病变的大鼠的基底前脑胆碱能神经元的变性和死亡 ,改善其学习记忆能力 。
Objective:Nerve growth factor transferrin conjugate(NGF Tf)was administered intravenously to Wistar rats, and we examined its effect on the Alzheimer's disease(AD) rats.Methods:NGF Tf was daily injected into Wistar rats with bilateral lesions of the cholinergic septo hippocampal pathway. We observed the rats' abilities of study and memory by using step down test and their ability of discriminating the direction by using water maze test. Septum and hippocampus were also conducted in morphological examination with light microscope. Result:In step down test, the number of errors in training time of the model group was obviously increased in comparison with those of the NGF Tf group(P<0 05) and normal group(P<0 01) ; in examining time, the number of errors of NGF Tf group was reduced by 50% compared with that of the model group. In water maze test, the average right reaction rate of arriving at terrace of NGF Tf group within 10 seconds was higher than that of the model group(P<0 05). Under microscope, Septum of NGF Tf group has a light withered change, but the nerve cells of model group in septum were markedly reduced;their outlines were blurred, and there were neuroglial cells proliferated; hippocampuses of normal control group and model group and NGF Tf group had no obvious pathological change.Conclusion:It is demonstrated that NGF Tf can pass through Blood brain barrier(BBB), effectively prevent the degeneration and death of cholinergic nerve cells in the AD rat basal forebrain, improve AD rats' abilities of study and memory, and promote the function of cholinergic nerve cells to revive.
出处
《广西医科大学学报》
CAS
2003年第2期173-176,共4页
Journal of Guangxi Medical University
基金
广西区科委资助项目
No.98110 3 9
9912 0 2 7
