摘要
目的 :探讨层粘连蛋白 (Ln)与佛波醇酯 (PMA)对人肝癌细胞粘连和增殖特性的影响及其相关机制 ,提供肝癌治疗的新线索。方法 :体外以人肝癌细胞系 (BEL - 74 0 2 )为靶细胞 ,在揭示靶细胞是否存在内源性Ln(enLn)表达的同时 ,利用促癌剂PMA(phorbol- 12 -myristate - 13-acetate)与外源性Ln(exLn)的作用来比较研究enLn及细胞蛋白激酶C -α(cPKC -α)活性表达变化与细胞粘连和增殖特性的关系。结果 :靶细胞呈现enLn的阳性表达 ,exLn能加强其细胞粘连及增殖能力 ;当PMA作用时 ,其细胞呈现enLn的更高程度表达 ,其细胞粘连性亦得到更明显地加强 ;而仅PMA的单独作用却能使其细胞呈现明显的增殖抑制 ,但PMA与exLn的共同作用 ,其细胞增殖力呈现在exLn单独作用得到加强的基础上获得更进一步的加强 ,显示出了协同效应。并且 ,PMA的单独作用能下调其细胞cPKC -α的表达 ,而外源性Ln的作用则能加强其细胞cPKC -α的表达。结论 :内、外源性Ln的作用与人肝癌细胞的粘连、增殖密切相关 ,其与癌细胞的cPKC -α的活性密切关联。
AIM: To explore the effects of PMA(phorbol-12-myristate-13-acetate,a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment. METHODS: Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigated in vitro. RESULTS: By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone,the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased. CONCLUSIONS: The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin ,which were associated with cPKC-α activity. Therefore,the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第7期927-929,T002,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目 (No .395 0 0 0 5 6 )
广东省"五个一兴教工程"基金资助项目
关键词
肝肿瘤
粘连
增殖
信号转导
层粘连蛋白
蛋白激酶C
佛波醇酯类
Liver neoplasms
Adhesions
Proliferation
Signal transduction
Laminin
Protein kinase C
Phorbol esters
