摘要
目的 探讨CD40-CD40配体(CD154)相互作用在血液透析患者免疫异常及肾脏损伤中的可能作用。方法 慢性肾功能衰竭进行血液透析患者22名,透析前采静脉血,正常对照10名,非空腹采静脉血;sCD40的测定采用ELISA法,CD4+T细胞上CD154的测定采用双色流式细胞仪。结果 sCD40在正常对照血浆中水平为(30.28±11.09)pg/ml,而血液透析患者为(781.22±203.03)pg/ml,显著高于正常对照(P<0.01)。外周血CD4+T细胞上CD154在正常对照的水平为(29.24±3.99)％,而血液透析患者为(41.41±5.54)％,也明显高于正常对照(P<0.05);说明在血液透析患者,其CD40-CD154相互作用处于过度活跃状态。结论 CD40-CD154可能通过影响免疫功能及加速肾脏损伤参与了尿毒症的发生发展,阻断CD40-CD154相互作用可能有助于延缓疾病进程。
Objective To investigate potential mechanisms by which CD40 - CD40 ligand (CD40L, CD154) interaction may be involved in the pathogenesis of immunodeficiency and renal injury in haemodialysis patients. Methods Soluble CD40 and CD154 on CD4 + T cells was detected in the serum of haemodialysis patients (n = 22) and healthy donors (n = 10) by the method of ELISA and two color flow cytometry, respectively. Results Soluble CD40 was detected in the serum of haemodialysis patients with a mean of(781.22±203.03)pg/ml,significantly higher than healthy donors(30.28±11.09)pg/ml, P < 0.01; CD154 on CD4 + T cells was detected in the serum of haemodial ysis patients with a mean of (41.41±5.54)%, elevated comparing with healthy donors (29.24±3.99)% , P<0.05. CD40 - CD40 ligand (CD40L) interaction may be actiatved in haemodialysis patients. Conclusion CD40 - CD154 may be involved in the pathogenesis of uremia by the means of immunodeficiency and renal injury.
出处
《中国血液净化》
2003年第7期363-365,共3页
Chinese Journal of Blood Purification
