摘要
目的 探讨核苷酸切除修复基因ERCCl(excisionrepaircross -complementing 1 )的表达与肺癌发病的关系。方法 用RT -PCR(reversetranscription -polymerasechainreaction)技术检测 1 50例肺癌组织和 50例正常肺组织中ERCCl基因的mRNA表达 ;免疫组化法检测ERCC1和癌变相关基因P53、C -MYC、K -RAS、BCL - 2和hTERT(端粒酶逆转录酶 )基因的蛋白表达 ;分析有关暴露因素对修复基因ERCCl表达的影响 ,并探讨ERCC1基因与P53、C -MYC、K -RAS、BCL - 2和hTERT等癌变相关基因的关系。结果 30 7% (46/ 1 50 )的肺癌组织和 4 0 % (2 / 50 )的正常肺组织存在ER CC1基因的表达低下 ;修复基因ERCC1表达低下与肺癌发生有关联 ,其危险度OR为 1 0 62 (2 38,65 98) ,P <0 0 0 1 ,人群归因危险度PARP为 8 87%。分析各种可能影响ERCC1基因表达的暴露因素 ,发现吸烟可抑制ERCC1基因的表达。另外发现 ,P53突变蛋白和K -RAS癌基因的过度表达与ERCC1表达低下有关。P分别为 0 0 388和 0 0 4 5 6。ERCC1与C -MYC、BCL - 2和hTERT基因的表达无关联。结论 ERCC1表达低下在肺癌发病中起着重要的作用 。
Objective To investigate the association between expression of nucleotide excision repair gene ERCC1(excision repair cross-complementing 1) and the development of human lung cancers.Methods The reverse transcription-polymerase chain reaction (RT-PCR) method was applied to measure ERCC1 mRNA expression in 150 cases of human lung cancer specimens and 50 normal lung tissues.The protein expressions of ERCC1 ,P53,C-MYC,K-RAS,BCL-2 and hTERT ( human telomerase reverse transcriptase ) were assessed by immuno-histochemistry.The effect of some exposure factors on the expression of ERCC1 gene were analyzed.The relationships between ERCC1 gene and cancer related genes P53,C-MYC,K-RAS,BCL-2 and hTERT were investigated.Results Reduced expression levels of ERCC1 were observed in 46 of 150 (30.7%) lung cancer specimens and 2 of 50 (4.0%) normal lung tissues.Low expression of DNA repair gene ERCC1 was associated with the development of human lung cancers,OR was 10.62 (95% CI 2.38-65.98), P <0 001,the population attributable risk proportion (PAR%) was 8.87%.In the investigation of the exposure factors that may affect the expression of ERCC1 gene.Smoking may suppress the expression of ERCC1 gene.The over-expression of P53 and K-RAS were correlated with the low expression of ERCC1 gene,P values were 0.0388 and 0.0456 respectively.But the expression of C-MYC,BCL-2 and hTERT were not correlated with the status of ERCC1.Conclusion Low expression of ERCC1 may play an important role in the development of human lung caners.And that low expression of gene could be used as a valuable susceptibility biomarker of human lung cancers.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2003年第7期780-782,共3页
Chinese Journal of Public Health
基金
国家自然科学基金资助 ( 30 2 0 0 2 35)
广东省重点科技攻关项目 ( 2 0 0 2B30 10 4
970 0 1)
广东省医学科研项目(B2 0 0 10 75)
