摘要
Microorganisms are commonly used to transform chemicals to chiral compounds. Pseudomonas cepacia 1813, Pseudomonas stutzeri 1317, Saccharomyces cerevisiae 1912, and 5 Escherichia coli strains were used to transform acetophenone to 2 phenylethanol. The results show that the E. coli strains have the poorest biotransformation ability. P. stutzeri 1317 provides the best transformation ability with a transformation rate of 30% achieved with either whole or broken P. stutzeri 1317 cells for a reaction pH of 8.2 and an initial acetophenone concentration of 2 g/L. An acetophenone concentration of 10 g/L strongly inhibits the biotransformation for a pH of 8.2. Crude alcohol dehydrogenase obtained from S. cerevisiae 1912 transforms acetophenone to 2 phenylethanol when nicotinamide adenine dinucleotide reduced (NADH) is added.
Microorganisms are commonly used to transform chemicals to chiral compounds. Pseudomonas cepacia 1813, Pseudomonas stutzeri 1317, Saccharomyces cerevisiae 1912, and 5 Escherichia coli strains were used to transform acetophenone to 2 phenylethanol. The results show that the E. coli strains have the poorest biotransformation ability. P. stutzeri 1317 provides the best transformation ability with a transformation rate of 30% achieved with either whole or broken P. stutzeri 1317 cells for a reaction pH of 8.2 and an initial acetophenone concentration of 2 g/L. An acetophenone concentration of 10 g/L strongly inhibits the biotransformation for a pH of 8.2. Crude alcohol dehydrogenase obtained from S. cerevisiae 1912 transforms acetophenone to 2 phenylethanol when nicotinamide adenine dinucleotide reduced (NADH) is added.
基金
Supported by the National Natural Science Foundation of China(No.2 0 0 74 0 2 0 )
