摘要
用酚水法从 2 0dm3 大黄鱼的病原菌———溶藻弧菌菌液分离出脂多糖 (LPS)溶液380cm3 ,冻干后得到LPS粉末 97.6mg .在注射剂量为 0 .2cm3 不同浓度LPS溶液对大黄鱼肌肉注射毒性试验中 ,LPS注射浓度为 0 .5mg/cm3 时 ,5d内能引起大黄鱼死亡 ;当LPS浓度提高到 2mg/cm3 时 ,大黄鱼的死亡率达到 1 0 0 % .以剂量为 0 .2cm3 、浓度为 0 .2mg/cm3 的LPS溶液对大黄鱼进行免疫注射 ,1 5d后用 0 .2cm3 浓度为 9× 1 0 8个 /cm3 的溶藻弧菌进行攻毒试验 ,4 0
97.6 mg LPS were obtained by phenol water method from 20dm 3 suspension of Vibrio alginolyticus ,the pathogenic bacteria of Pseudosciaena crocea. Toxicity of LPS from V.alginolyticus on P.crocea was examined by intramuscular injection.The results showed that injecting 0.5mg/cm 3 LPS would lead to the death of P.croceain 5d, and the mortality rate of P.crocea would reach 100% when the concentration of LPS raised to 2.0mg/cm 3. 15d after injection of LPS (0.2mg/cm 3) 0.2cm 3 to P.crocea, 40% of P.crocea were immunely protected when attacked by 0.2cm 3 V.alginolyticus suspension cells (9×10 8ind./cm 3).
出处
《台湾海峡》
CAS
CSCD
2003年第2期163-167,共5页
Journal of Oceanography In Taiwan Strait
基金
国家高技术研究发展计划 ( 86 3 819 2 0 12)
福建省科技厅大黄鱼养殖病害专项资助项目
关键词
酚水法
大黄鱼
病原菌
溶藻弧菌
脂多糖
死亡率
免疫保护
毒理学
LPS
marine fishery
toxicology
phenol water method
Vibrio alginolyticus
LPS
Pseudosciaena crocea
immune protection
