摘要
以冻干法制备胶原 壳聚糖多孔膜 ,测定其细胞毒性 ,种植人真皮成纤维细胞 (Fbs)并观察其增殖情况 ,用ELISA法测定人工真皮上清液IL 6、IL 8的浓度 ,观察经冻存复苏的人工真皮Fbs的增殖情况及体外酶降解速率等方面研究胶原 壳聚糖活性人工真皮。认为研制的胶原壳聚糖多孔膜有良好的细胞相容性 ,能在体外构建活性人工真皮 ,初步证实能用冻存的方法保存活性人工真皮。
To study living artificial dermis, we synthesized collagen-chitosan porous membrane by freeze-drying method, measured its cytotoxicity, implanted human dermal fibroblasts (Fbs) on to it, and observed the proliferation state of Fbs. We also measured IL-6, IL-8 contents in the supernatant of the artificial dermis by ELISA method, observed the proliferation state of Fbs on the cryopreserved membrane, and measured the degrading velocity of the porous membrane in the presence of collagenase. The collagen-chitosan porous membrane was good in cell compatibility and the coryopreservation method could be used to construct living artificial dermis in vitro.
出处
《中国生物医学工程学报》
EI
CAS
CSCD
北大核心
2003年第4期358-363,共6页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金资助项目 ( 5 0 173 0 2 4)
关键词
人工真皮
胶原
壳聚糖
Biological membranes
Biosynthesis
Cells
Collagen
