摘要
目的探讨生脉成骨方对内毒素致血管内皮细胞(EC)损伤的影响。方法从兔主动脉取材进行EC培养,分别用内毒素和生脉成骨方含药血清对培养的EC进行处理,通过光镜、电镜及MTT法观察EC形态结构及功能的变化。结果内毒素对EC能产生病理性损伤,而生脉成骨方含药血清组细胞形态基本正常;MTT法结果提示,内毒素对EC的增殖具有抑制作用,减弱其功能活性;而生脉成骨方含药血清能增加EC的代谢活性,促进其增殖。结论生脉成骨方对内毒素导致的EC损伤有保护作用。
Objective To observe the effect of Shengmai Chenggu Prescription (SCP) on the damage of endothelial cells (EC) induced by endotoxin. Methods EC obtained from rabbit's aorta were cultured and were treated with endotoxin and serum containing SCP respectively. Histological changes and function of the cultured cells were observed under light microscope and electron microscope and with MTT method . Results Endotoxin could result in the pathologic injury of cultured EC and inhibit the proliferation of the cells. Serum containing SCP could increase the activity of EC and promote its proliferation. Conclusion SCP can protect endothelial cells from damage induced by endotoxin.
出处
《中药新药与临床药理》
CAS
CSCD
2003年第4期227-230,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金课题(编号30171143)
教育部重点资助项目(编号200055)
广东省自然科学基金课题(编号010251)
广东省中医药局课题(编号102013)
关键词
@生脉成骨方/药理学
细胞培养
内毒素/副作用
血管内皮细胞/药物作用
Shengmai Chenggu Prescription/pharmacology
Cell culture
Endotoxins/adverse effects
Vascular endothelial cells/drug effects
