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阳离子膜融合脂质体包裹DNA体外转染和稳定性研究 被引量：3

Studies on transfection efficiency of cationic fusogenic liposomes mediated DNA and stability of the complexes
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摘要 目的　研究阳离子膜融合脂质体包裹DNA的体外稳定性以及体外细胞转染。方法　采用表达E .coilβ 半乳糖苷酶的pSV4 0 β报告基因质粒及组化染色评价阳离子膜融合脂质体的转染效率。用DNase 1考察阳离子膜融合脂质体的体外稳定性。结果　当阳离子膜融合脂质体 (CFL)及阳离子脂质体 (CL)与DNA电荷比为 (2∶1)时 ,CFL的转染效率为 (4 2 .3±4 .3) % ,明显高于CL的转染效率 (2 3.9± 2 .1) % ,且有很较高的稳定性。结论　CFL可作为载体有待进一步研究。 OBJECTIVE. To evaluate the transfection efficiency of LacZ reporter gene mediated with cationic fusogenic liposomes (CFL) and the stability of CFL/DNA complexes in present of DNase I. METHODS. The cationic liposomes(CL) were prepared by reverse-phase evaporation vesicles. The cationic fusogenic liposomes were obtained through fusing with UV-inactivated Sendai virus, and the complexes were formed by physical absorption. Agarose gel electrophoresis was used to observe the stability of CFL/DNA complexes incubating with DNase I at different time. Hela cells were transfected with LacZ reporter gene by CL and CFL. Transfection efficiency was estimated with histochemical staining of Hela cells for β-galactosodase activity. RESULTS. The transfection efficiency of the two vectors were (23.9 ± 2.1) % and (42.3 ± 4.3) % respectively as +/- charge ratio was 2:1. The DNase I protection assay of CFL/DNA complexes showed the stability. CONCLUSION. The study demonstrates the ability of CFL to improve gene transfection efficiency and stability against DNase I.
作者 胡英 金一 潘小平
机构地区 浙江大学药学院 浙江大学医学院附属一院传染病研究所
出处 《中国药学杂志》 EI CAS CSCD 北大核心 2003年第7期547-549,共3页 Chinese Pharmaceutical Journal
基金 国家自然科学基金项目 (No :93 0 0 70 896)
关键词 阳离子膜融合脂质体 包裹DNA 体外转染 稳定性 研究 Bioassay Complexation DNA Genetic engineering Viruses
分类号 Q523 [生物学—生物化学]
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参考文献8

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二级参考文献11

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共引文献7

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二级引证文献45

中国药学杂志
2003年 第7期

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