摘要
目的:观察采用在膻中穴注射鱼腥草法对哮喘豚鼠进行治疗的效果,探讨穴位注射法在防治哮喘方面的作用途径和机制,为防治此病提供新的思路。方法 :将50只荷兰种雄性豚鼠随机分成5组,即空白对照组、模型对照组、鱼腥草穴位注射组、鱼腥草非穴位注射组和生理盐水穴位注射组。采用卵蛋白致敏和激发法建立哮喘豚鼠的模型,在为各组豚鼠进行穴位注射治疗后经肉眼观察其哮喘症状发作的程度,记录其引喘潜伏期。结果 :鱼腥草穴位注射组豚鼠激发后的哮喘症状比鱼腥草非穴位注射组豚鼠和生理盐水穴位注射组豚鼠更轻,鱼腥草非穴位注射组豚鼠和生理盐水穴位注射组豚鼠的哮喘症状相比较无明显的差异性。与鱼腥草非穴位注射组豚鼠和生理盐水穴位注射组豚鼠相比,鱼腥草穴位注射组豚鼠的引喘潜伏期明显较长,差异显著(P<0.05)。与鱼腥草非穴位注射组豚鼠、生理盐水穴位注射组豚鼠相比,鱼腥草穴位注射组豚鼠的哮喘症状明显减轻(P<0.05)。结论 :对哮喘豚鼠采用膻中穴注射鱼腥草注射液法进行治疗可有效延长其引喘潜伏期,明显改善其哮喘症状发作的程度,取得良好的疗效。
Objective: To observe the therapeutic function of the tanzhong(RN17) point-injection in asthmatic guinea pigs in lung tissue, so as to discuss the functionary approach and mechanism of point-injection in asthmatic prevention and treatment, for providing prevention and treatment of asthma with new ideas and better methods, and for laying certain foundation for the further study about the functionary mechanism of point-injection method in asthmatic treatment. Methods: In experiment , fifty male Holland guinea pigs were randomly divided into five groups with ten guinea pigs in each group: the blank control group, the model control group, the point injecting houttuynia group, the non-point injecting houttuynia group and the point injecting saline group. we succeed in copying the classical animal model of asthmatic guinea pigs with ovalbumin. After treatment of point-injection, we note the latent period of asthma, observe the degrees of asthma attack . Results: Tanzhong point-injection effectively extend the latent period of asthma and relieve acute attack of asthma symptoms. The result of PMN numbers in BALF can show: it can significantly decrease the PMN numbers, restrain the PMN infiltration and alleviate airway inflammation in guinea pigs with asthma. Conclusions: Tanzhong point-injection can evidently improve the outbreak degrees of asthmatic symptoms and has pronounced the effect on guinea pigs with asthma.Futher more,the point injecting houttuynia group has the best effect.
关键词
支气管哮喘
穴位注射
Bronchial asthma,Point injection
