摘要
目的研究铁负荷对大鼠脂质过氧化的影响及具有抗氧化作用的维生素 (维生素E和 β 胡萝卜素 )对铁负荷所产生影响的作用。方法将 80只雄性Wistar大鼠按体重随机分为 8组 ,前 4组给予的饲料铁浓度分别为 5 0、2 0 0、3 5 0和 5 0 0mg kg饲料。后 4组饲料铁浓度分别与前 4组相同 ,但同时在饲料中补充抗氧化剂(维生素E 10 0mg kg饲料、β 胡萝卜素 2 5mg kg .BW) ,喂养 8周。实验结束时 ,测定血清铁、血脂 (总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇 )、维生素E、维生素A ,血清丙二醛、氧化性低密度脂蛋白 ,超氧化物歧化酶和谷胱甘肽过氧化物酶。结果铁负荷具有增加血清丙二醛、氧化性低密度脂蛋白及低密度脂蛋白胆固醇水平的趋势 ,5 0 0mg kg饲料组显著 ;各组谷胱甘肽过氧化物酶活性应激性增加 ,VE、VA含量下降。加入维生素类抗氧化剂可使丙二醛、氧化性低密度脂蛋白及低密度脂蛋白胆固醇降低 ,高密度脂蛋白胆固醇升高 ,超氧化物歧化酶降低。结论铁负荷可促进体内脂质过氧化反应 ,以十倍生理剂量最为显著 ,同时抗氧化酶类活性增加 ,以维持机体动态平衡 ;维生素类抗氧化剂可能通过非酶促反应体系发挥作用 。
In order to explore the influences of iron overload on lipid peroxidation of rats and the inhibiting effects of antioxidant vitamins such as vitamin E and β-carotene on the influences induced by iron overload, eighty male Wistar rats were randomly divided into 8 groups according to the weight. The diets of the former 4 groups differed only in iron concentration (50, 200, 350 or 500mg/kg diet). The diet of the latter 4 groups were consistent with the former 4 groups respectively, but complemented with vitamin E(100mg/kg diet) and β-carotene(25mg/kg·BW) at the same time. After Eight weeks, the levels of serum iron, blood lipid including total cholesterol TC, triglyceride TG, high density lipoprotein HDL, low density lipoprotein LDL, vitamin E, vitamin A, serum maglonydiadehyde (MDA) and oxidized low density lipoprotein(OX-LDL), the activities of superoxide dismutase (SOD) and glututhione peroxidase (GSH-Px) were determined. Results showed that the iron overload increased the levels of MDA, OX-LDL and LDL in serum, especially in the group of rat with 500mg/kg diet. The activity of GSH-Px increased irritably and the contents of Vitamin E and Vitamin A decreased significantly. Complementing antioxidant vitamins decreased the levels of MDA, OX-LDL and LDL and increased HDL and the activity of GSH-Px decreased compared with the corresponding iron overload groups. It was concluded that iron overload could promote lipid peroxidation reaction especially in 10 folds of physiologic dose and the activities of antioxidant enzymes increased at the same time to keep the body homeostasis. Antioxidant vitamins may play a role through non-enzymatic reactive system to inhibit the above changes.
出处
《卫生研究》
CAS
CSCD
北大核心
2003年第3期209-211,共3页
Journal of Hygiene Research
