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神经鞘瘤的分子遗传学研究 被引量：2

The study of molecular genetics of schwannomas
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摘要 目的:分析神经鞘瘤22号染色体杂合子丢失(CHR22 LOH)及其与临床行为之间的关系。方法:通过变性聚丙烯酰胺凝胶、银染观察神经鞘瘤CHR22 LOH频率;通过Ki-67、PCNA免疫组织化学计算神经鞘瘤的增殖指数。结果:36例神经鞘瘤中15例发生CHR22 LOH(41.6％),发生缺失的神经鞘瘤的增殖指数高于无缺失者(P<0.05)。结论:CHR22 LOH是神经鞘瘤发生中的常发事件,缺失的区域包括NF2基因,CHR22 LOH可能是神经鞘瘤增殖的重要因素。 Objective: To analyze the loss of heterozygosity for markers on 22 chromosome (CHR22 LOH) and the relationship between it and clinic behavior. Methods: We observed the frequency of CHR22 LOH by denatured polyacry-lamide gels and silver staining. The proliferative index of schwannoma was calculated by Ki-67, PCNA immunohis-tochemistry. Results: 15 schwannomas(41. 6%) showed allele loss. The proliferative index of schwannomas with LOH were significantly higher than those without LOH (P<0. 05). Conclusion: CHR22 LOH was the frequent event in the tumorigenesis of sporadic schwannoma, including NF2 gene. The CHR22 LOH might be an important factor to affect the proliferation of schwannoma.
作者 孙青芳 卞留贯 赵卫国 沈建康
机构地区 上海第二医科大学附属瑞金医院神经外科
出处 《脑与神经疾病杂志》 2003年第4期205-208,共4页 Journal of Brain and Nervous Diseases
基金 上海市科委启明星课题(00QB142024) 上海市教委课题(2000QN86)资助
关键词 神经鞘瘤 分子遗传学 22号染色体 杂合子丢失 基因突变 Schwannoma Microsatteiite markers Loss of heterozygosity
分类号 R730.264 [医药卫生—肿瘤] Q75 [生物学—分子生物学]
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参考文献11

  • 1National Institutes of Health] National Institudes of Health Consensus Development Conference, Neurofibromatosis 1 (Recklinghausen disease) and neurofibromatosis 2 (bilateral acoustic neurofibromatosis). Am Intern Med, 1990,113(1) :39.
  • 2Troffater JA, MacCollin MM, Rulter JL, et al. A novel moesinezrin-, radixin-like gene is a candidate for the neurofibromatosis type 2 tumor suppressor. Cell, 1993,72 (1) : 1.
  • 3Rouleau GA, Merel P, Lutchman M, et al. Alterations in a new gene encoding a putative membrane-organising protein causes neurofibromatosis type 2. Nature, .1993,363, (5) : 515.
  • 4Marineau C, Aubery M, Julien JP, et al. Dinucleotide repeat polymorphism at the D22S264. Nucleic Acids Research, 1992,20(6):1430.
  • 5Marineau C, Baron C, Delattro O, et al. Dinucleotide repeat polymorphism at the D22S268 locus. Hum Mol Genet, 1993,2(3):336.
  • 6Sainio M, Strachan T, Blomstedt G, el al. Presymptomatic DNA and MRI diagnosis of neurofibromatosis 2 with mild clinical course in a exterded pedigree. Neurology, 1995,45(7):1314.
  • 7Sainz J, Baser ME, Ragge NK, et al. Loss of alleles in vestibular schwannomas: use of microsatellite markers on chromosome 22.Arch Otolaryngol Head Neck Surg, 1993,119(12):i285.
  • 8Budowle B, Chakraborty R, Giusti AM, et al. Analysis of the VNTR locus DIS80 by the PCR followed by high-resolution PAGE.Am J Hum Genet, 1991,48(2):137~144.
  • 9Irving RM, Moffat DA, Hardy DG, et al. Molecular genetic analysis of the mechanism of tumorigenesis in acoustic neuroma. Arch Otolaryngol Head Neck Surg, 1993,119(11):1222.
  • 10Moffat DA, Irving RM. The molecular genetics of vestibular schwannoma. J Laryngol Otol, 1995,109(3):381.

同被引文献19

  • 1范同英,孙运铎.一家四代七例椎管肿瘤[J].中华神经外科杂志,2004,20(5):408-408. 被引量：1
  • 2卞留贯,孙青芳,沈建康,罗其中.多发神经纤维瘤病的临床和分子生物学研究[J].中华神经外科疾病研究杂志,2005,4(5):477-479. 被引量：10
  • 3BIAN Liu-guan,SUN Qing-fang,Tirakotai Wuttipong,ZHAO Wei-guo,SHEN Jian-kang,LUO Qi-zhong,Bertalanffy Helmut.Loss of heterozygosity on chromosome 22 in sporadic schwannoma and its relation to the proliferation of tumor cells[J].Chinese Medical Journal,2005(18):1517-1524. 被引量：8
  • 4LEE H, KIM D, DAN H C, et al. Identification and characterization of putative tumor suppressor NGB, a GTP-binding protein that interacts with the neurofibromatosis 2 protein [J]. Mol Cell Biol, 2007, 27(6): 2103-2119.
  • 5LEE D J, MASEYESVA B, WESTRA W, et al. Microsatellite analysis of recurrent vestibular schwannoma (acoustic neuroma) following stereotactic radiosurgery [J]. Otol Neurotol, 2006, 27(2): 213-219.
  • 6MENTEN B, PATTYN F, DE PRETER K, et al. ArrayCGHbase: an analysis platform for comparative genomic hybridization microarrays [J]. BMC Bioinformatics, 2005, 6: 124.
  • 7MANTRIPRAGADA K K, BUCKLEY P G, BENETKIEWICZ M, et al. High-resolution profiling of an 11 Mb segment of human chromosome 22 in sporadic schwannoma using array-CGH [J]. Int J Oncol, 2003, 22(3): 615-622.
  • 8HASEGAWA M, FUJISAWA H, HAYASHI Y, et al. Surgi- cal pathology of spinal schwannomas: a light and electron microscopic analysis of tumor capsules [J]. Neurosurgery, 2001, 49(6): 1388-1393.
  • 9WARREN C, JAMES L A, RAMSDEN R T, et al. Identification of recurrent regions of chromosome loss and gain in vestibular schwannomas using comparative genomic hybridisation [J]. J Med Genet, 2003, 40(11): 802-806.
  • 10BEDAVANIJA A, BRIEGER J, LEHR H A, et al. Association of proliferative activity and size in acoustic neuroma: implications for timing of surgery [J]. J Neurosurg, 2003, 98 (4): 807-811.

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脑与神经疾病杂志
2003年 第4期

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