摘要
以 1 2 2 8个品种与V41A测交F1的花粉育性为依据 ,划分品种恢保类型及系谱分析 ;验证生产上应用的和成型不育系的育性动态变化 ;确定保持系细胞核中r基因效应 ;用 4个配组模式选育保持系转育新不育系 ,探讨淘汰技术方法 ,实践总结认为 :( 1 )我国南方矮仔占衍生的大量籼稻品种含有r基因 ,与不育系测交F1出现黑染花粉率在 0 .1 %～ 1 0 %之间 ,但这些品种遗传基础丰富 ,是选育不育系的重要种质资源 ;( 2 )r基因干扰育性遗传 ,表现在不育系出现花粉嵌合现象 ,不育系出现嵌合花粉囊越多说明保持系细胞核中含r基因数量越多 ;( 3 )要选育遗传性稳定的不育系 ,首先要选育好的保持系 ;( 4 )保持系细胞核中r基因的淘汰关键世代是在保持系F2 ～F5,不育系的B1F1～B4F1。
Based on F 1 pollen fertility in 1228 varieties and V41A test cross, varietal restorer-maintainer types were divided, pedigree were analysed, the fertility dynamics of male sterile (MS) line applied to production was tested and verified, and the minor restorer gene(r) effect of cell nucleus of maintainer line was determined. Maintainer lines were bred and transferred to select new MS lines by crossing the exclusive technological methods, which was practiced for over ten years. The results are as follews: The F 1 in many indica varieties crossed with the MS line caused 0.1%～10% black dye pollen rate. These varieties are important germplasm resources for selecting MS lines owing to their ample genetic base; the genetic effect of r gene on fertility interference shows pollen mosaic phenomenon in the line, and the more mosaic pollen sacs the larger r gene numbers from the cell nucleus of the maintainer line; in breeding MS lines with stable genetic characters, the key generations are F 2 to F 5 of the maintainer line and B 1F 1 to B 4F 1 of the MS line.
出处
《江西农业大学学报》
CAS
CSCD
2003年第4期487-492,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
福建省重大科技项目---优质籼型三系不育选育与种子产业化 ( 2 0 0 2N0 0 8)
