摘要
目的观察预防性应用氟桂利嗪(商品名西比灵)对实验性脑缺血再灌注细胞凋亡及神经细胞损伤的保护作用。方法取沙土鼠按随机数字表法分为4组:假手术组,缺血再灌注组,西比灵预防组,西比灵治疗组。采用加闭双侧颈总动脉法复制沙土鼠脑缺血再灌注模型。假手术组除不夹闭颈总动脉外,其余操作与其他组相同;西比灵预防组于脑缺血再灌注前后各3d给予西比灵10mg/kg,1次/d,连续6次;西比灵治疗组于脑缺血再灌注术后给予西比灵10mg/kg,1次/d,连续3次;术后3d处死动物取材,光、电镜观察神经细胞形态,测脑组织超氧化物歧化酶(SOD)活力,原位末端缺中翻译标记法检测凋亡细胞。结果预防性应用西比灵组与其他3组相比,细胞缺血性形态损伤明显减轻,脑组织SOD活力明显增高犤(31.0±5.8)Nu/mg犦(P<0.01),凋亡细胞表达明显受抑制(40.6±3.3)个(P<0.05)。结论预防性应用西比灵能明显减轻缺血再灌注对神经细胞的损伤。
Aim To observe the preventive effects of sibelium (SBL) in protecting experimental cerebral ischemia reperfusion cell against apoptosis and injuries as an experimental support of SBL in extensive clinical application.Methods Thirty two gerbils were equally divided into four groups at random: sham operation group(group A), ischemia reperfusion group (group B), SBL prevention group (SBL were used before and after ischemia reperfusion, group C) and SBL treatment group (SBL were used only after ischemia reperfusion, group D). Cerebral ischemia replicas of the gerbils were made by clamping closed bilateral common carotid artery in the group B, C and D except group A. Every animal of the SBL prevention group received SBL of 10 mg/kg once a day for three days before and after ischemia reperfusion respectively, and the duration of treatment was six days. In the SBL treatment group, every animal received SBL of 10 mg/kg once a day for three days after the ischemia reperfusion only. All the animals were killed at three days after operation. The section preparations were observed by optical microscope. The nerve cell structures of the ultra thin section were observed by electron microscope. And the superoxide dismutase (SOD) activity of the cerebral tissues and cell apoptosis were measured.Results The activity of SOD in the SBL prevention group was (39.8± 4.8)NU/ mg, significantly lower than that of the sham operation group[(2.3± 6.0)NU/mg], but significantly higher than that of the ischemia reperfusion group(31.0± 5.8)NU/mg]0.05),buthigherthanthatoftheshamoperationgroup(25.7±4.2).ConclusionSBLpreventsnervecellinjuryinducedbyischemiareperfu-sionsignificantly.
出处
《中国临床康复》
CSCD
2003年第19期2652-2653,共2页
Chinese Journal of Clinical Rehabilitation
