摘要
目的 :了解临床下呼吸道感染标本常见革兰阴性杆菌分离株中AmpC酶和超广谱 β内酰胺酶 (ESBLs)的检出情况。方法 :通过酶提取物三维试验检测单产AmpC酶、同时产AmpC酶和ESBLs菌株 ;采用美国国家临床实验室标准委员会 (NC CLS)表型筛选和确认试验检测单产ESBLs菌株。结果 :从临床下呼吸道标本分离对第一、二代及一种以上第三代头孢菌素耐药的 2 2 6株常见革兰阴性杆菌 ,包括阴沟肠杆菌、弗劳地枸橼酸杆菌、肺炎克雷伯菌、大肠埃希菌、鲍曼不动杆菌和铜绿假单胞菌中 ,分别检出单产AmpC酶、同时产AmpC酶和ESBLs及单产ESBLs细菌 34株、1 5株、1 0 9株 ,总检出率分别为 1 5 .0 %、6 .6 %、4 8.2 %。AmpC酶检出率在阴沟肠杆菌中最高 ,为 5 7.9% ;ESBLs检出率以肺炎克雷伯菌最高 ,其次为大肠埃希菌 ,分别为 78.5 %、77.8%。结论 :下呼吸道产AmpC酶和ESBLs细胞感染常见 ,前者以阴沟肠杆菌为主 。
Objective: The prevalence of AmpC β lactamases and extended spectrum β lactamases (ESBLs) producing strains were investigated from specimens of lower respiratory tract. Methods: AmpC β lactamases producing and AmpC β lactamases combined with ESBLs producing strains were detected by three dimensional test, ESBLs producing isolates were identified using method recommended by the National committee for Clinical Laboratory Standard (NCCLS). Results: Among 226 clinical isolates of Gram negative bacilli including E. cloacae, C. freundii, K. pneumoniae, E. coli, A. baumannii and P. aeruginosa , resistant to the first , second and at least one third generation cephalosporins, AmpC β lactamases, AmpC β lactamases combined with ESBLs, and ESBLs producing strains were found in 34, 15 and 109 strains, respectively, the incidence being 15.0%, 6.6% and 48.2%, respectively. AmpC β lactamases was detected in 57.9% of E. cloacae ESBLs detected in 78.5% of K.pneumoniae and 77.8% of E. coli. Conclusions: AmpC β lactamases and ESBLs are common in clinical strains of lower respiratory tract infections, the former mainly existed in E. cloacae strains and the latter in mainly K. pneumoniae and E. coli. strains.
出处
《中国抗感染化疗杂志》
2003年第4期220-222,共3页
Chinese Journal of Infection and Chemotherapy
关键词
AMPC酶
超广谱Β内酰胺酶
下呼吸道
AmpC β lactamase
Extended spectrum β lactamase
Lower respiratory tract
