摘要
我们应用rIL-2从乙肝患者肝穿组织中获得59个T细胞克隆,其CD_4^-CD_3^+克隆占71.19%,HLA-DR^+占93.22%。并对CD_4^-CD_3^+克隆的各种细胞毒活性进行了分析,结果表明,来自乙肝患者肝组织的CD_4^-CD_3^+T克隆的细胞介导的细胞毒(CTL)活性明显增高(P<0.001),而NK及ADCC活性与来自原发性胆汁性肝硬化患者肝组织及正常人外周血的CD_4^-CD_3^+T克隆相比差异无显著性(P>0.05),提示在乙肝的持续性肝损伤中,CD_4^-CD_3^+T细胞亚群及CTL活性可能起着重要作用。
Fifty-nine clones obtained by limiting dilution by Using rIL-2
from liver biopsies of 3 patients with cHB. of the 59 clones 42 were of
the 'cytotoxic/suppressor' T cell subset(CD8) and 55 of 59 clones
expressed HLA-DR--related antigens, that was they were in an activated
state. Functional studies on 42 clones(CD8) revealed high cytotoxic
activity of all of these clones in a LDCC assay reflecting the killing
capacity of CTL, and comparing in NK and ADCC cytotoxicity assays
no differrence were demonstrated with the T cell clones from liver
tissue of patients with primary biliary cirrhosis and from PBL of
healthy individuals.
出处
《临床肝胆病杂志》
CAS
1992年第2期61-63,共3页
Journal of Clinical Hepatology
