摘要
目的:探讨酸枣仁汤对抑郁大鼠大脑皮质星形胶质细胞胶质原纤维酸性蛋白(Glial fibrillary acidic protein,GFAP)和缝隙连接蛋白43 (Connexin43,Cx43)的作用。方法:将80只大鼠随机分为空白组、模型组、氟西汀组和中药组4组,每组20只;除空白组外,以慢性不可预见性温和应激(Chronic unpredictability mild stress,CUMS)结合孤养建立大鼠抑郁模型;各组分别进行相应处理,对各组大鼠糖水消耗情况、旷场实验指标变化情况、大脑皮质神经阻滞细胞损伤情况及大脑皮质GFAP、Cx43表达情况等进行比较。结果:与空白组比较,模型组大鼠糖水消耗量、水平及垂直活动均明显减少,但神经损伤总评分、大脑皮质星形胶质细胞GFAP、Cx43表达均明显升高,差异均有统计学意义(P <0.05);与模型组比较,氟西汀组及中药组大鼠2组糖水消耗量、水平及垂直活动均明显增多,神经损伤总评分、脑皮质星形胶质细胞GFAP、Cx43表达均明显减小,差异均有统计学意义(P <0.05);中药组与氟西汀组各检测指标差异均无统计学意义(P> 0.05)。结论:酸枣仁汤可显著减轻抑郁大鼠糖水消耗量及大鼠神经细胞损伤情况,减弱其自主活动,并降低其GFAP、Cx43表达。
Objective:To discuss the effect of Suanzaoren tang on glial fibrillary acidic protein(GFAP)and connexin 43(Cx43)in astrocytes in cerebral cortex in rats with depression.Methods:A total of 80 rats were randomly divided into the blank group,the model group,the fluoxetine group and the Chinese medicine group,20 rats in each group.Except those in the blank group,the rat models with depression were established by chronic unpredictability mild stress(CUMS)and social isolation in the other groups.Each group was treated accordingly,and the glucose consumption,the changes in indexes of the Open-Field Test,the cell injury in cerebral cortex after never block and the expressions of GFAP and Cx43 in cerebral cortex in each group were compared.Results:Compared with those in the blank group,the glucose consumption as well as the horizontal and vertical movements in the model group were significantly decreased,while the total scores of never injury and the expressions of GFAP and Cx43 in astrocytes in cerebral cortex in the model group were significantly increased,differences being significant(P<0.05).Compared with those in the model group,the glucose consumption as well as the horizontal and vertical movements in the fluoxetine group and the Chinese medicine group were significantly increased,while the total scores of never injury and the expressions of GFAP and Cx43 in astrocytes in cerebral cortex in the two groups were significantly decreased,differences being significant(P<0.05);there was no significant difference being found in the comparison of each indexes between the fluoxetine group and the Chinese medicine group(P>0.05).%Conclusion:Suanzaoren tang can significantly reduce the glucose consumption,the injury of neurons and the expressions of GFAP and Cx43,and weaken the locomotor activity in rats with depression.
作者
刘克洪
孙建伟
胡晓华
LIU Kehong;SUN Jianwei;HU Xiaohua
出处
《新中医》
CAS
2019年第10期13-16,共4页
New Chinese Medicine
