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大鼠脑中催产素受体cDNA的克隆

大鼠脑中催产素受体cDNA的克隆
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摘要 目的 :克隆大鼠脑中催产素受体 (OTR)cDNA编码区全长。方法 :从分娩后SD大鼠脑组织中提取总RNA ,通过RT -PCR扩增出大鼠OTRcDNA ,与pMD18-T载体连接 ,构成重组质粒 ,进行测序并与子宫中OTRcDNA序列进行比较。结果 :获得OTRcDNA编码区全长 ,并且与Rozen等报道的子宫OTRcDNA序列完全一致。结论 :克隆了脑中催产素受体 (OTR)cDNA ,并且证明中枢神经系统内OTR与外周的OTR一样。
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2003年第8期1109-1110,1128,共3页 Chinese Journal of Pathophysiology
基金 国家自然科学基金资助项目 (No .395 70 811)
关键词 受体 催产素 基因 Receptors, oxytocin Brain Genes
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参考文献6

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