摘要
噬菌斑平板计数是微生物学理论研究与实际应用中常用的方法之一。但由于平板上噬菌斑与背景反差小,而且往往出现几个噬菌斑相连,计算机识别时出现较大的误差,因此噬菌斑计数目前仍为人工方法。本文以λ噬菌体为材料,感染E.coli宿主细胞,获得噬菌斑。然后将噬菌斑制成电子图像。抽取图像中有代表性的区域,利用分水岭算法对图像进行分割处理,将相连的噬菌斑分割成单独的噬菌斑,然后利用基于区域生长法进行计数,结果与人工计数完全相同,表明我们建立的新方法可以用于噬菌斑计算机自动计数。
Plaques counting is a routine method in the theory research and practiced application of microbiology. Because of weak contrast between plaque and the background of the plate, and the plaques linking together, a serious error was produced using automatic counting by computer. So that plaques have been counted by artificial method. In the present study, a new method was established for automatic counting plaques by computer. At first, The plaques were formed by infecting Escherichia coli with λ bacteriophage, and prepared as the method of soft-agar layer plate. The electrical images counted the plaques were obtained by screening the plaques plates. A representative area, in which the plaques were linked each other, was picked out from each image, and handed with watershed algorithm. The plaques linking together were separated into individual plaque alone in the images. And then, the images were counted with the method of growth algorithm based on area by computer. The exact effect of counting plaques was in agreement with artificial method. It indicates the new method of counting plaques can be used in automatic counting by computer.
出处
《中国病毒学》
CSCD
2003年第4期387-390,共4页
Virologica Sinica
关键词
噬菌斑
电子图像
计算机处理
自动计数
Handle image
Watershed algorithm
Automatic counting
Growth algorithm based on area.
