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抗癌药莪棱舌草Ⅱ号诱导人肺癌细胞凋亡的研究 被引量:2

TCSs′ Effect on Apoptosis of Human Lung Cancer Cell Line A549
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摘要 通过运用流式细胞光度术检测细胞凋亡 ;运用细胞化学方法检测凋亡相关基因蛋白的表达以及Westernblotting分析等方法来研究抗癌药莪棱舌草Ⅱ号对人肺癌细胞 (A5 4 9细胞 )凋亡的诱导作用。结果表明药物能够诱导A5 4 9细胞发生凋亡 ,凋亡率近 40 % ;药物作用可以使细胞中凋亡抑制基因bcl 2表达减少 ,而凋亡促进基因Bax表达增加。提示诱导肿瘤细胞凋亡可能是莪棱舌草Ⅱ号抑杀肺癌细胞的机制之一。 TCSs, one kind of Chinese traditional herbs, is the serials of anticancer medicine. The purpose is to study the mechanism of tumor inhibitory and killing effects of the TCSs on lung cancer cell line A549. With methods of flow cytometry and Western blotting, we investigated tumor cell apoptosis induced by the herb. It also examined the expression of apoptotic protein with cytochemistry method. The results indicated that the herb can induce tumor cell apoptosis, and the apoptotic rate can reach to 40 percent. After being treated by the herb, the expression of antiapoptosis gene bcl-2 reduced, and proapoptosis gene Bax increased. The results suggest that to induce target cells apoptosis is the main mechanism to inhibit and kill tumor by the herb. ;
出处 《中国生物工程杂志》 CAS CSCD 2003年第8期74-76,共3页 China Biotechnology
基金 北京市青年科技骨干培养基金资助项目
关键词 抗癌 莪棱舌草Ⅱ号 诱导 人肺癌细胞 细胞凋亡 TCSs Human lung cancer cell Apoptosis
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参考文献7

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二级参考文献2

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共引文献41

同被引文献13

  • 1余元龙,季明芳,何洁冰,李晓玲.紫杉醇对肝癌细胞增殖抑制和诱导凋亡的效应研究[J].中华实验外科杂志,2004,21(7):877-878. 被引量:6
  • 2孙斌,瞿伟菁,张晓玲,杨煌建,庄秀园,章平.蒺藜皂苷对人肝癌细胞BEL7402生长抑制和诱导凋亡作用的研究[J].中国中药杂志,2004,29(7):681-684. 被引量:17
  • 3张志勉,钟宁,高海青,张尚忠,魏媛,辛华,梅行,侯怀水,林希蕴,时庆.大蒜素对皮下移植肝癌细胞生长抑制和诱导凋亡的实验研究[J].中华老年医学杂志,2005,24(3):213-216. 被引量:7
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