摘要
目的 :从油菜蜂花粉中提取总黄酮 ,并测定其含量。方法 :采用超声技术提取总黄酮 ,用比色法测定总黄酮含量。结果 :油菜蜂花粉中总黄酮含量为 (30 .5 0± 1.0 0 ) % ,平均回收率为 99.6 0 % ,RSD =2 .95 % (n =6 )。结论 :运用超声技术从油菜蜂花粉中提取总黄酮 ,可提高提取率 ,节省提取时间。
Objective: To extract and determine chromoco of bee pollen of rape. Methods: Ultrasonic technology was aplied to extracting chromoco and the content was determined with colormetry. Results: The chromoco content was 31.00%,recovery rate was 99.60%. RSD=2.95%. Conclusion: Ultrasonic technology can improve extraction rate of chromoco in bee pollen of rape, and can save extraction time.
