摘要
本文介绍了利用生物发光技术测定靶细胞释放出的ATP浓度来计算NK细胞活性的方法。实验中发现效应细胞在培养过程中细胞内ATP自然释放现象;培养后细胞内ATP浓度(3.4±0.21)比培养前低(4.7±0.3),P<0.05。对细胞内ATP提取方法做简要探讨,以细胞冻融法较理想。同时对25名中、晚期肿瘤病人和50名献血员NK细胞活性进行了测定,结果肿瘤病人NK活性(11.7±4.5)明显低于对照组(26.5±3.0),P<0.01。该法测定NK活性较为简便并避免了同位素半衰期短和放射性污染等缺点。
A new method was established to detect periphral NK cell activity by determining the ATP concentration released from target cell using bioluminescence technology. ATP was found to be spontaneously released from effect cells during culture. The intracellular ATP concentration after calture was significantly lower than that before (P<0. 05). Method to extract ATP from cells was also briefly discussed. It's clinical application showed that the NK cell activity of 25 patients with middle -later stage digestive cancers was significantly lower than that of 50 normal blood doners,being 11. 7±4. 5vs and 26. 5±3. 0%(P<0. 01). Besides eliminating isotopes,this method was quite easy to perform.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1992年第4期260-262,共3页
Immunological Journal
