摘要
目的 测定不同储存条件下氯霉素滴眼液的稳定性。方法 高效液相色谱法 ,采用ZORBAXTM -C18色谱柱 (4 .6×15 0mm) ,粒径 5 μm ;流动相 :0 .0 1mol·L-1NaH2 PO4-甲醇 -乙腈 (30 0∶16 0∶4 0 ,加三乙胺 0 .5ml,用磷酸调pH3.0 ) ,流速 :1.0ml/min ,检测波长 :2 78nm ,内标法峰高定量。结果 氯霉素在 10～ 5 0 μg·ml-1范围内 ,其浓度与峰高比呈良好的线性关系 ,回归方程为Y =0 .0 30 398X +0 .0 5 10 8,r =0 .9995。平均回收率为 10 0 .2 5 % ,RSD为 0 .94 %。结论 氯霉素滴眼液宜冰箱冷藏保存 ,贮存时间一年。
Aim To study the stability and expiration of chloramphenicol eye drops.Methods The contents of chloramphenicol and its related substances were determined by means of HPLC.Chromatogrophic conditions included YWS-C 18 column and the mobile phase consist of 0.01mol·L -1 NaH 2PO 4-methanol-acetonitril(300∶160∶40).The detective wavelength was 278nm.Results The calibration curves were linear in the range of 10～50μg·ml -1 .The average recovery was 100.25%(RSD=0.94%).Conclusion Chloramphenicol eye drops may be stored for 1 year at cold storage.
出处
《解放军药学学报》
CAS
2003年第4期264-266,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
