摘要
目的 :探讨甲胎蛋白 (AFP)异质体检测对原发性肝癌、良性肝病的临床应用价值。方法 :根据小扁豆凝集素对AFP亲和力不同 ,采用亲和免疫电泳印迹法检测AFP L3;化学发光免疫分析法检测AFP。结果 :以AFP L3≥ 15 %作为PHC的诊断标准 ,其敏感性为 91 1% ,与慢性肝病鉴别诊断的特异性为 95 .0 % ,AFP L3含量与血清AFP浓度及原发性肝癌癌灶大小无关。结论 :AFP L3结合AFP检测对原发性肝癌 ,尤其是小肝癌的早期诊断和与良性肝病的鉴别诊断具有重要的临床应用价值。
Objective:To study the clinical value of lectin reactive alpha fetoprotein(AFP) detection in primary hepatocellular carcinoma (PHC) and benign liver diseases.Methods:According to the different affinity with AFP, AFP L 3 was detected by affinity immunoelectrophoresis blotting method, AFP detected by Chemiluminescent Immunoassay.Results:Taking AFP L 3≥15% as the diagnosis criteria of PHC,the sensitivity was 91.1%,the specificity in differential diagnosis in chronical liver diseases was 95.0%,the levels of AFP L 3 has no correlation to serum AFP levels and the sizes of PHC.Conclusion:It is of great clinical value to detect AFP L 3 and serum AFP levels for PHC in early diagnosis and benign differential diagnosis in liver diseases. [
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2003年第9期651-653,共3页
Chinese Journal of Immunology
基金
浙江省台州市科委科技项目 (No .0 0 2 2 0 9)资助
关键词
AFP异质体
检测
临床应用
原发性肝癌
小扁豆凝集素
Lectin reactive alpha fetoprotein
Primary hepatocellular carcinoma
Leng culinaris agglulinin
