摘要
建立了中药中补骨脂素、异补骨脂素的高效液相色谱分析方法。采用的色谱条件为:Symmetry C18色谱柱(4.6mm×250mm,5μm),用乙腈-水(30:70)作为流动相,检测波长为246 nm,流动相流速为1.0m L/min,柱温为35℃。补骨脂素和异补骨脂素的浓度在3~30μg/m L时,组分的峰面积与浓度之间的线性关系良好,补骨脂素的线性方程为A=26983C+12603(C单位:μg/m L),R^2=0.9998,异补骨脂素的线性方程为A=29208C+10084(C单位:μg/m L),R^2=0.9997,回收率在97.6%~103.3%,精密度良好,补骨脂素、异补骨脂素的RSD值分别为0.718%、0.810%。方法简便,快速,适用于补骨脂素和异补骨脂素的同时测定,中药补骨脂和中成药驱白巴布期片中补骨脂素和异补骨脂素含量存在差异。
A high performance liquid chromatographic method was established for simultaneous determination of psoralen and isopsoralen in Chinese herbal medicine. The optimum chromatographic conditions were: Symmetry C18 column( 4. 6mm ×250mm,5μm),acetonitrile and water( 30: 70) as mobile phase,detection wavelength for 246 nm,1. 0m L/min as the flow rate,35℃ as the column temperature. The peak area and concentration showed a good linear relationship,when psoralen and isopsoralen concentration was in the range of 3 ~ 30μg/m L. The linear equation of psoralen was A = 26983 C + 12603( C: μg/m L),R^2= 0. 9998,and that of isopsoralen was A = 29208 C + 10084( C: μg/m L),R^2= 0. 9997. The recoveries of target compounds in Chinese herbal medicine were in the range of 97. 6% ~ 103. 3%. Relative standard deviation( RSD) of psoralen and isopsoralen was 0. 718% and 0. 810%,the precision of the method was good. The method was simple,rapid,and was used for the simultaneous determination of psoralen and isopsoralen. There are differences of psoralen and isopsoralen content between fructus psoraleae and Qubai Babuqi Tablet.
出处
《山东化工》
CAS
2016年第18期55-57,共3页
Shandong Chemical Industry
基金
忻州师范学院大学生科技创新项目(院政字2015-88)
忻州师范学院青年基金项目(QN201519)
忻州师范学院应用化学创新实践基地(2013-31)资助
