摘要
目的制作兔骨髓间充质干细胞(BMSCs)-骨形态蛋白2(BMP2)-煅烧牛骨复合物,并观察其在兔尺骨骨缺损修复中的应用。方法将兔BMSCs分离、培养及成骨诱导后,接种于BMP2-煅烧牛骨复合物,扫描电镜下观察复合情况。将54只新西兰大白兔随机分为观察组、对照组、空白组各18只,于无菌条件下造成右侧尺骨中段10mm长缺损。观察组植入BMSCs-BMP2-煅烧牛骨复合物,对照组植入BMP2-煅烧牛骨复合物,空白组不植入任何材料,术后均不做任何内外固定。各组术后4、8、12周每组随机处死6只,分别行骨缺损处的放射学和组织学检查。结果成骨诱导后的BMSCs均匀分布在煅烧骨表面及孔洞内,与煅烧骨表面贴附紧密,细胞呈圆形,表面光滑,胞体较大,直径15μm。随着术后时间的延长,观察组和对照组Lane-Sandhu放射学评分、组织学评分均逐渐升高,P均<0.05;空白组无变化,P均>0.05。术后4、8、12周,观察组和对照组Lane-Sandhu放射学评分、组织学评分均高于空白组,观察组升高更明显,P均<0.05。结论 BMSCs能够较好地与BMP2-煅烧牛骨复合物进行体外复合培养,BMSCs-BMP2-煅烧牛骨复合物植入有助于兔尺骨骨缺损的修复。
Objective To make the rabbit bone marrow mesenchymal stem cells( BMSCs) / bone morphogenetic protein 2( BMP2) / calcined bone complex,and to observe its application in the repair of bone defect of rabbit ulna. Methods After the isolation,culture and osteogenic induction of rabbit BMSCs,we inoculated on BMP2 / calcined bone complex,and observed the compound situation under scanning electron microscope. Fifty-four New Zealand white rabbits were randomly divided into the experimental group,control group,and blank group with 18 rats in each group. Under the sterile conditions,10 mm defect of the right middle ulna was made. The experimental group was implanted BMSCs / BMP2 / calcined bone complexes,while the control group was implanted BMP2 / calcined bone complexes,and the blank group without any implantation. After the operation,we did not make any internal and external fixation. After operation 4,8,and 12 weeks,6 rabbits in each group were randomly sacrificed,and the radiological and histological examinations of the bone defects were conducted. Results The BMSCs cells after the osteogenic induction uniformly distributed in the bone surface and hole,and calcined bone surface attached closely,cells were round with smooth surface,large cell body and the diameter of 15 μm. With the extension of time,the Lane-Sandhu radiological score and histological score of the experimental group and the control group were increased gradually( all P < 0. 05); the blank group had no change( P > 0. 05). Four,eight and twelve weeks after implantation,the Lane-Sandhu radiological score and histological score of the experimental group and the control group were higher than those of the control group,and the experimental group increased more significantly( all P < 0. 05). Conclusion BMSCs can co-culture with BMP2 / calcined bone complexes better in vitro,and the implantation of BMSCs / BMP2 / calcined bone complexes help the repair of rabbit ulna bone defect.
出处
《山东医药》
CAS
北大核心
2015年第12期17-20,111,共5页
Shandong Medical Journal
基金
湖北省卫生厅科研指导性项目(JX6C-44)
关键词
骨髓间充质干细胞
骨形态蛋白2
煅烧牛骨
骨缺损
兔
bone marrow mesenchymal stem cells
bone morphogenetic protein 2
calcined bone
bone defect
rabbits
