期刊文献+

肺宁颗粒对放射性肺损伤大鼠细胞因子表达的影响 被引量:1

Effect of Feining granule on expression of cytokines in rats with radiation-induced lung injury
下载PDF
导出
摘要 目的探讨肺宁颗粒防治大鼠急性放射性肺损伤(radiation induced lung injur,RILI)及对细胞因子转化生长因子β1(transforming growth factor-β1,TGF-β1)、白介素-1(interleukin-1,IL-1)、白介素-6(interleukin-6,IL-6)的影响。方法选取105只雌性SD大鼠,根据随机数字表法将大鼠分成7组,分别为正常组、模型组、地塞米松组(阳性药物)、肺宁颗粒低剂量组、肺宁颗粒中剂量组、肺宁颗粒高剂量组、肺宁颗粒预防组,每组各15只。除去正常组,余各组均全胸接受X线单次照射15 Gy,DT30Gy/2f/1w。肺宁颗粒预防组于放射治疗前1周起开始灌胃肺宁颗粒;余组在照射后48 h起灌胃。于2、4、6周分别采用随机法每组取出5只大鼠,处死后取右肺,采用免疫组化法检测放疗后不同时段各组大鼠肺组织TGF-β1、IL-1、IL-6的含量。结果放疗后第2、4、6周模型组大鼠肺组织TGF-β1、IL-1、IL-6强表达,显著高于正常组(P<0.05)。经肺宁颗粒干预后各时间点大鼠肺组织中TGF-β1、IL-1、IL-6表达显著低于同时点模型组(P<0.05),且呈一定浓度依赖性。但肺宁颗粒高、中、低剂量组TGF-β1、IL-1、IL-6表达均不同程度高于地塞米松组(P<0.05),而肺宁颗粒预防组上述指标表达低于地塞米松组(P<0.05)。结论肺宁颗粒能够通过降低TGF-β1、IL-1、IL-6的表达防治放射性肺损伤。肺宁颗粒各治疗组疗效不如地塞米松,但肺宁颗粒预防的疗效优于地塞米松。 Objective To investigate Feining granule on prevention and treatment of rat with radiation-induced lung injur ( RILI) and its effect on cytokine transforming growth factor-β1(TGF-β1), interleukin-1 (IL-1), interleukin-6 (IL-6).Methods 105 SD female rats were selected, according to random number table, and divided into seven groups: normal group, model group, dexamethasone group(positive drug), Feining granule low-dose group (FN low-dose group), FN medial-dose group, FN high-dose group and FN prevention group, 15 rats in each group.Except for normal group, all remaining groups received the X-ray irradiation of 15Gy, DT30Gy/2f/1w.FN prevention group were intragastric infused with FN granule one week before irradiation, and the other groups 48 h after irradiation.Five rats were sacrificed randomly at 2, 4, 6 weeks respectively, and right lung tissues were taken out.The contents of TGF-β1, IL-1 and IL-6 were detected by immunohistochemical method.ResuIts TGF-β1, IL-1 and IL-6 contents in lung tissue of model group at 2, 4, 6 weeks were higher than those of normal group (P<0.05).The above indicators after treated by Feining granule were lower than those of model group at each time (P<0.05),with a concentration-dependence manner to some extent.The above indicators in FN high-, medial-and low-dose group were higher than those in dexamethasone group (P<0.05), to some extent.However, the above indicators in FN prevention group were lower than those in dexamethasone group ( P<0.05 ) .ConcIusion Feining granule could prevent and cure radiation induced lung injury through decreasing TGF-β1, IL-1 and IL-6 content.The efficacy of dexamethasone is stronger than FN treatment groups, but is weaker than FN prevention.
出处 《中国生化药物杂志》 CAS 2015年第8期23-26,30,共5页 Chinese Journal of Biochemical Pharmaceutics
基金 贵州省中医药管理局(QZYY2010-66)
关键词 放射性肺损伤 肺宁颗粒 转化生长因子-Β1 白介素-1 白介素-6 radiation-induced lung injury Feining granule transforming growth factor-β1 interleukin-1 interleukin-6
  • 相关文献

参考文献2

  • 1Indira Madani,Kim De Ruyck,Hannelore Goeminne,Wilfried De Neve,Hubert Thierens,Jan Van Meerbeeck.Predicting Risk of Radiation-Induced Lung Injury[J]. Journal of Thoracic Oncology . 2007 (9)
  • 2Rube C E,Uthe D,Schmid K W,Richter K D,Wessel J,Schuck A,Willich N,Rube C.Dose-dependent induction of transforming growth factor beta (TGF-beta) in the lung tissue of fibrosis-prone mice after thoracic irradiation. International journal of radiation oncology, biology, physics . 2000

同被引文献23

引证文献1

二级引证文献7

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部