摘要
目的建立高效液相色谱(high performance liquid chromatography,HPLC)测定血管微透析样品中拉莫三嗪含量的方法,测定拉莫三嗪微透析体外回收率并考察其影响因素,筛选适用于体内实验的微透析条件。方法采用正、反透析法测定拉莫三嗪血管微透析的体外回收率,并考察流速、浓度、温度、时间对回收率的影响,验证反透析法用于测定体内回收率的可行性并筛选出体内实验的最佳灌流速度和平衡时间;采用反透析法测定血管探针的体内回收率并考察其体内稳定性。结果在相同条件下探针相对回收率与相对损失率近似相等,并随流速增加而降低,随温度升高而增高;在相同流速下,回收率与药物浓度无关;当灌流速度为2μL/min,平衡时间为1.5 h时,探针的体内回收率可维持6.5 h稳定。结论微透析采样技术可用于拉莫三嗪的血药浓度研究,反透析法可作为拉莫三嗪血管微透析体内回收率的测定方法。
Objective To establish a high performance liquid chromatography method to detect the concentration of lamotrigine in blood dialysate and investigate in vitro recovery of lamotrigine and the factors. Select the microdialysis conditions that apply to the animal experiment and guide the stability study of in vivo recovery. Methods Positive dialysis and retrodialysis were used for the examination of lamotrigine in vitro recovery and the influencing factors such as flow rate,concentration,temperature and time. Filtered out the best conditions that apply to the in vivo experiment. Used the retrodialysis to determine the in vivo recovery and its stability. Results There was no significant difference between relative recovery and relative loss in the same flow rate. The concentration had no obvious effect on relative recovery. At the same condition,relative recovery decreased with the increase of the flow rate and increased with the temperature. The in vivo recovery had a good stability of 6. 5 hours when the flow rate and stabilization time were set at 2 μL / min and 1. 5 h,respectivily. Conclusion Microdialysis technique can be used for the pharmacokinetic study of lamotrigine. Retrodialysis can be used for the determination of the lamotrigine in vivo recovery.
出处
《中国生化药物杂志》
CAS
2015年第10期122-126,共5页
Chinese Journal of Biochemical Pharmaceutics
基金
广东省省级科技计划项目(2013B021800091)
关键词
拉莫三嗪
微透析
体外回收率
体内回收率
lamotrigine
microdialysis
in vitro recovery
in vivo recovery
