摘要
为了解新疆部分地区近年来绵羊肺腺瘤病(Ovine pulmonary adenomatosis,OPA)的流行病学特征,对新疆6个地区部分种羊场进行调查,提取肺脏组织和血液中的DNA,经临床诊断和病理学观察并用PCR法对样品进行检测。结果表明:病变肺脏组织中为外源性OPA的阳性率是36.36%(4/11),血样中为内源性OPA的阳性率是78.25%(1981/2534)。外源性阳性样品env基因与ex JSRV核苷酸同源性最近的是内蒙株(JQ837489)为98.1%,与en JSRV核苷酸同源性最近的是美国株(EF680300)为86.5%,进化树分析结果可知本实验ex JSRV和en JSRV两者存在地域性。这说明新疆部分地区内源性OPA感染较普遍,而外源性OPA感染相对较少。本研究可为新疆地区OPA的防制提供理论依据。
To investigate the epidemiological characteristics of Ovine pulmonary adenomatosis(OPA) in Xinjiang area.DNA was extracted from the blood and the lung tissue of the Sheep from six areas in Xinjiang,and furthertested by PCR and clinical pathological observation,The results showed that the positive rate of exogenous OPA in lung tissue lesions was 36.36%(4/11), the positive rate of endogenous OPA in blood samples was 78.25%(1981/2534).Nucleotide homology of env gene in ex JSRV was98.1% compared with InnerMongolia strains(JQ837489),and when compared with American strain(EF680300)en JSRV was 86.5%.The amplified fragment was sequenced forPhylogenetic tree analysis,it was found that the ex JSRV and en JSRV were regional existed.It was concluded that the endogenous OPA infection was common in parts of Xinjiang,while the exogenous OPA infection was relatively few.This study provided a theoretical basis forthe prevention of OPA in Xinjiang.
出处
《石河子大学学报（自然科学版）》
CAS
2015年第5期568-573,共6页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金项目(30960276
31360589)
关键词
绵羊肺腺瘤病毒
流行病学
调查
jaagsiekte sheep retrovirus(JSRV)
epidemiology
survey
