摘要
目的通过体外药效评价重楼皂苷Ⅰ对肺癌循环肿瘤细胞(circulating tumor cells,CTC)增殖、凋亡的影响。方法分别用不同浓度的重楼皂苷Ⅰ干预CTC细胞24 h、48 h、72 h后,采用CCK-8法检测细胞增殖情况,采用流式细胞术和激光共聚焦显微镜分析CTC凋亡与周期情况。结果重楼皂苷Ⅰ能够显著抑制CTC增殖并具有浓度依赖性,诱导CTC细胞核形态发生明显改变,使凋亡细胞的比例显著升高,并将CTC细胞的周期阻滞在G0/G1期。结论重楼皂苷Ⅰ通过诱导CTC凋亡,抑制细胞从G0/G1期向S期转化,从而发挥抗肿瘤作用。
Objective To evaluate the effects of Polyphyllin I on proliferation and apoptosis of circulating tumor cells( CTC) of lung cancer in vitro. Methods The CTC were intervened by Polyphyllin I at different concentrations for 24,48 and 72 hours. The cell proliferation was determined by CCK-8,and the cell apoptosis and cycle were detected by flow cytometry and confocal laser scanning microscopy. Results Polyphyllin I could significantly inhibit the CTC proliferation in a concentration-dependent manner,and induce the significant morphological changes of CTC nucleus. The proportion of apoptotic cells was significantly increased,and the cells cycle was stagnated in G0/G1 phase. Conclusion Polyphyllin I can induce CTC apoptosis and inhibit the cells differentiation from G0/G1 to S phase,which plays an anti-tumor effects.
出处
《上海中医药杂志》
2017年第5期77-81,共5页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(81373621)
上海中医药大学预算内项目(2015YSN37)
上海市卫生局中医药事业发展三年行动计划项目(ZY3-CCCX-2-1002)
关键词
重楼皂苷Ⅰ
肺癌循环肿瘤细胞
细胞增殖
细胞凋亡
细胞周期
polyphyllin Ⅰ
circulating tumor cells of lung cancer
cell proliferation
cell apoptosis
cell cycle
