摘要
巴氏蘑菇(AgaricusblazeiMurill)菌丝体经热水提取、乙醇沉淀、脱蛋白和透析得总多糖,再经DEAE-纤维素(DE52)和sephadexG 200柱层析分离纯化得均一多糖,凝胶渗透色谱法测得其相对分子量为8 6×104。红外和紫外光谱分析表明为蛋白多糖,含蛋白质28 8%,含糖68 4%。纸层析和气相色谱分析表明,该蛋白多糖的单糖组成为葡萄糖、甘露糖和半乳糖,其摩尔比为12 64∶2 16∶1 28。
A proteoglycan was isolated and purified from cultured mycelia of Agaricus blazei Murill by hot water,ethanol precipitation,protein removal(sevage method),dialysis,DEAECellulose column chromatography and Sephadex G200 gel filtration.It was proved to be homogenous by polyacrylamide gel electrophoresis,and with the molecular weight of about 86×104 by gel filtration chromatography.As identified by IR and UV,it was a polysaccharideprotein complex with 684?% carbohydrate and 288?% protein.Identified by paper chromatography and gas chromatography,it was proved that the proteoglycan was composed of glucose,mannose,and galactose with a molar ratio of 1264∶216∶128.
出处
《西南农业学报》
CSCD
2003年第3期30-33,共4页
Southwest China Journal of Agricultural Sciences
