摘要
通过酸性平板筛选,在作者所在实验室保藏的运动发酵单胞菌中,得到一株能在pH4.5时进行酒精发酵的菌株,其酒精产量为66.7g/L,与原菌株在pH6.5时的相同.通过单因素实验和正交实验,对该菌在酸性条件下的酒精发酵条件进行了优化.结果表明,在发酵温度为30～35℃,pH4.5,初糖质量浓度150g/L,酵母膏质量浓度4g/L,蛋白胨质量浓度为2g/L时,该菌的酒精产量达到最大,为71.7g/L.
An acidresistant strain of Zymomonas mibilis with ethanol yield of 71.7 g/L was selected from the acid flat. It could produce the same amout of ethanol as the original strain did in the acid condition of pH 4.5. Fermentation conditions such as substrate concentration, temperature, pH were optimized in this paper. Based on the orthogonal experiment, the optimum fermentation media was found as the followings: 150 g/L gluculose, 4 g/L yeast extraction, 2 g/L peptone, and pH 4.5.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2003年第5期76-79,共4页
Journal of Wuxi University of Light Industry
基金
江苏省科委基金项目(BQ98039)资助课题.
