摘要
目的筛选小麦和其他相关食品中常见的赭曲霉毒素A真菌毒素核酸适配体。方法采用固态靶标筛选策略和磁分离的筛选方法筛选赭曲霉毒素A的核酸适配体。结果获得的赭曲霉毒素A核酸适配体亲和力较高,解离常数为纳摩尔级;特异性好,与赭曲霉毒素A结构相似性化合物如赭曲霉毒素B、N-乙酰苯丙氨酸或华法令不结合或结合力非常弱。结论本文采用固态靶标筛选策略和磁分离的SELEX筛选方法获得了赭曲霉毒素A的高特异性、高亲和力核酸适配体,有望在农产品质量安全领域替代传统抗体开发真菌毒素快速检测传感器或制备固相亲和柱。
Objective To screen aptamer of ochratoxin A(OTA) in wheat and other related food. Methods The solid-target selection strategy and magnetic-separation selection method were adopted to screen aptamer of ochratoxin A. Results The OTA aptamer had high affinity with nanomolar dissociation constants and good specificity non-recognizing, and it had weak affinity towards structure similarity compounds of OTA such as Ochratoxin B(OTB), N-acetyl-L-phenylalanine and warfarin. Conclusion The OTA aptamer that got from solid-target selection strategy and magnetic-separation selection method, has high affinity and good specificity. Instead of traditional antibody, the OTA aptamer may be used to develop sensor and affinity chromatography column for mycotoxins rapid detection in the field of agricultural products.
出处
《食品安全质量检测学报》
CAS
2015年第9期3543-3550,共8页
Journal of Food Safety and Quality
基金
国家公益性行业(农业)项目(201203094)
国家自然科学基金项目(21305031)
农业部重点项目(2011-G5)~~
