摘要
目的建立快速检测牛奶中双甲脒的气相色谱法。方法试样中的双甲脒经酸水解成2,4-二甲基苯胺,碱化后用正己烷提取,七氟丁酸酐衍生,气相色谱-电子捕获检测器检测,外标法定量。结果该方法检测双甲脒回收率范围在83.6%~97.4%之间,相对标准偏差在1.02%~4.23%之间,最低检出限为5μg/kg。结论该方法简单、快速、分离度好,适用于牛奶中双甲脒含量的测定。
Objective To develop a method for rapid determination of amitraz content in milk by gas chromatography(GC). Methods The amitraz in a sample was hydrolyzed into 2,4-dimethylaniline by acid, and reacted with alkalinity. The resultant was extracted by N-hexane, and then derived by heptafluorobutyric anhydride. The derivatives were detected and quantified by GC with electron capture detector and external standard method, respectively. Results The recoveries of amitraz were 83.6%~97.4%, the relative standard deviation were 1.02%~4.23%, and the detection limit was 5 μg/kg. Conclusion This method is simple, rapid, and has a good separating degree, and it is suitable for the determination of amitraz in milk.
出处
《食品安全质量检测学报》
CAS
2015年第10期4225-4229,共5页
Journal of Food Safety and Quality
关键词
双甲脒
牛奶
气相色谱
毛细管色谱柱
amitraz
milk
gas chromatography
capillary-column chromatography
