摘要
建立HPLC法同时测定维药菊苣茎、根、种子总黄酮提取液中芦丁、槲皮素和山柰酚含量的方法。用安捷伦HC-C_(18)柱(250 mm×4.6 mm, 5μm)色谱柱,流动相为0.1%乙腈(A)-磷酸(B)(50︰50, V/V),流速0.8 mL/min,检测波长365 nm,柱温30℃。结果表明,芦丁、槲皮素和山柰酚分别在2.0～20μg/mL (r^2=0.999 9), 0.5～40μg/m L (r^2=0.999 8)和0.5～60μg/m L (r^2=0.999 1)浓度范围内呈现良好线性关系,加标回收率(n=3)分别为97.7%(RSD=1.89%), 97.3%(RSD=1.01%)和102.6%(RSD=0.33%)。菊苣茎中芦丁含量为116.3×10~3μg/kg,槲皮素为122.4×10~3μg/kg,山柰酚为32.7×10~3μg/kg;菊苣根中存在芦丁,含量为82.0×10~3μg/kg;菊苣子中存在芦丁和槲皮素,芦丁含量为85.0×10~3μg/kg,槲皮素为45.3×10~3μg/kg。该方法简便、快速、有效、灵敏、准确,具有良好回收率,可作为该植物的定量分析方法,试验结果可为以后菊苣资源的开发利用提供依据。
The content of rutin,quercetin and kaempferol in extract of total flavonoids of stem,root and seed of Cichorium intybus L.was determined by HPLC.HPLC with HC-C18 column(250 mm×4.6 mm,5μm),and the mobile phase was0.1%CH3 CN,H3 PO4(50︰50,V/V)for gradient elution,flow rate 0.8 mL/min,detection wavelength 365 nm,and column temperature 30℃.The result showed that the linear responses were ranged from 2.0-20μg/m L for rutin(r2=0.999 9),0.5-40μg/m L for quercetin(r2=0.999 8),and 0.5-60μg/m L for kaempferol(r2=0.999 1),respectively.The average recoveries of rutin,quercetin and kaempferol were 97.7%(RSD=1.89%),97.3%(RSD=1.01%)and 102.6%(RSD=0.33%).The content of rutin in stems was 116.3×103μg/kg;The quercetin was 122.4×103μg/kg;The kaempferol was 32.7×103μg/kg;The content of rutin in roots was 82.0×103μg/kg;The content of rutin in seeds was 85.0×103μg/kg;The quercetin was 45.3×103μg/kg.The method was simple,rapid,effective,sensitive and accurate,with good recovery rate,which could be used as a quantitative analysis method for the plant.The experimental results might provide important theoretical basis for the future development and utilization of cichorium resources.
作者
热阳古.阿布拉
夏娜
姑丽各娜.买买提依明
木合塔尔.吐尔洪
REYANGGU·Abula;XIA Na;GULGINA·Mamtimin;MUHETAER·Tu’erhong(College of Chemistry and Environmental Sciences-Kashgar University,Kashgar 844000;Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry,Kashgar 844000)
出处
《食品工业》
CAS
北大核心
2019年第3期267-270,共4页
The Food Industry
基金
国家自然科学基金项目(项目编号:21665013)
喀什大学校内课题青年专项(项目编号:(14)2524)
