摘要
设计建立测定海洋鲈鱼鱼油中EPA (二十碳五烯酸)和DHA (二十二碳六烯酸)含量的方法,并测定尿素包合前后鱼油中EPA和DHA含量变化。采用Agilent色谱柱型号XDB (C_(18)),设置柱温38℃、检测波长220 nm、恒定流速1 mL/min,流动相乙腈-水梯度洗脱。结果表明, EPA甲酯和DHA甲酯的检测线性范围分别为0.059～5.9和0.094～9.4μg (R^2_(EPA)=0.999 6, R^2_(DHA)=0.999 9),检出限分别为0.5和0.9μg/mL。在精密度试验中, EPA甲酯和DHA甲酯的RSD分别为0.29%和0.34%。在加样回收率试验中,分别设计了6个加标浓度做检测, EPA甲酯和DHA甲酯的平均回收率分别为99.29%～99.87%(平均99.52%)和99.59%～101.48%(平均100.22%),平均RSD分别为0.24%和0.83%。尿素包合前,原料鱼油中EPA和DHA的含量分别为1.23%和2.52%;尿素包合后,含量分别为5.66%和12.34%。建立的HPLC法稳定、快捷、重复性好,适用于海鲈鱼下脚料鱼油中EPA和DHA含量测定。尿素包合后EPA和DHA的含量显著增长,达到包合前的4.8倍。
To establish a method for determination of EPA and DHA in sea bass oil,the concentrations before and after urea adduction were determined.Agilent XDB C18 column was used to determine contents.Components were eluted gradiently,and acetonitrile-water’s flow velocity was designed at constant 1.0 mL/min,while the column’s temperature was unchanging38℃,and the wavelength of UV detection was set at 220 nm.The EPA and DHA’s linear ranges were 0.059-5.9(R2=0.999 6)and 0.094-9.4μg(R2=0.999 9),respectively;The limits of detection were 0.5 and 0.9μg/mL,respectively.In the precision experiment,RSDs were 0.29%and 0.34%for EPA and DHA.In the sample recovery experiment for 6 different concentrations of adding sample,the recoveries’average values are 99.52%for EPA(RSD=0.24%)and 100.22%for DHA(RSD=0.83%).The contents of EPA and DHA are 1.23%and 2.52%respectively before urea adduction,5.66%and 12.34%after urea adduction.The method was simple,rapid,accurate and reproducible for the detection of EPA and DHA in sea bass oil.The concentrations of EPA and DHA increase rapidly.After urea adduction,the total concentration become 4.8 folds of raw oil’s.
作者
张凌云
杨莹
邓世明
ZHANG Lingyun;YANG Ying;DENG Shiming(College of Oceanography,Hainan University,Ministry of Education Key Laboratory of Tropic Biological Resources(Haikou 570228))
出处
《食品工业》
CAS
北大核心
2019年第4期327-330,共4页
The Food Industry
基金
"十二五"国家科技支撑计划项目"热带海洋特色生物资源开发与利用"(2013BA01B04)
