摘要
为研究羊栖菜褐藻糖胶的结构及其抗氧化活性,以羊栖菜为原料,经CaCl_2溶液提取、DEAE-Sepharose fast flow分离纯化得到褐藻糖胶组分SFP-2。采用高效凝胶渗透色谱法(HPGPC)、化学法、高效液相法(HPLC)以及傅里叶变换红外光谱法(IR)对多糖的纯度、分子量、理化性质、单糖组成以及官能团进行测定,并通过测定DPPH自由基清除活性、超氧阴离子清除活性、ABTS^+自由基清除活性、亚硝酸盐清除活性和还原力,对多糖的体外抗氧化活性进行了评价。结果表明,SFP-2分子量均一,为707.0 kDa;总糖含量为66.9%,蛋白含量为3.1%,硫酸基含量为21.2%,不含糖醛酸; SFP-2主要由岩藻糖和半乳糖构成,还含有少量的甘露糖和氨基葡萄糖;红外光谱显示,SFP-2具有硫酸化多糖常见官能团的特征吸收峰;上述结果表明SFP-2是一种不含糖醛酸的硫酸化岩藻聚糖。SFP-2具有良好的清除DPPH自由基、超氧阴离子、ABTS+自由基、亚硝酸盐活性,其EC50分别为8.94、8.30、16.04、9.87 mg/mL,且具有一定的还原能力。因此,SFP-2是一种不含糖醛酸的,抗氧化活性良好的羊栖菜褐藻糖胶。
In order to study the structural characterization and antioxidant activities of fucoidan from Sargassum fusiforme,a heterogeneous polysaccharide SFP-2 was obtained by CaCl2 solution extraction and anion-exchange.Structural characterization of SFP-2 was preliminarily determined by high performance gel permeation chromatography( HPGPC),chemical methods,high performance liquid chromatography(HPLC) as well as fourier-tansform infrared(IR).The antioxidant activities were evaluated by scavenging assays involving DPPH radical,superoxide anion,ABTS+ radical,nitrite and reducing power. Results indicated that SFP-2 appeared as a single and symmetrical peak in the HPGPC chromatogram and the average molecular weight was 707.0 kDa.The contents of total sugar,protein and sulfate ester were 66.9%,3.1% and 21.2%,respectively.No uronic acid was detected,SFP-2 was mainly composed of fucose and galactose with a small amount of mannose and glucosamine. IR further proved SFP-2 was a sulfated polysaccharide.From the results,SFP-2 was a sulfated fucoidan without uronic acid.SFP-2 had good scavenging activity for DPPH radical,superoxide anio,ABTS+ radical and nitrite.The EC50 values of the scavenging assays were 8.94,8.30,16.04,9.87 mg/mL,respectively,with the ability of reducing power.Results above indicated that SFP-2 was a kind of non-glucuronic acid fucoidan which had better antioxidant activity.
作者
刘雪
王桂宏
赵福江
周鑫
Mikhail Kusaikin
刘昌衡
LIU Xue;WANG Gui-hong;ZHAO Fu-jiang;ZHOU Xin;Mikhail Kusaikin;LIU Chang-heng(Biology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103,China;Yantai Dajing Ecological Environment Technology Co.,Ltd.,Yantai 264003,China;Shandong Jibeier Bio-Technique Co.,Ltd.,Jinan 250014,China;Laboratory of Enzyme Chemistry,G.B.Elyakov Pacific Institute of Bioorganic Chemistry,Far-Eastern Branch of the Russian Academy of Sciences,Vladivostok 690022,Russia)
出处
《食品工业科技》
CAS
北大核心
2019年第3期79-84,共6页
Science and Technology of Food Industry
基金
国家重点研发计划(2018YFC0311200)
山东省重点研发计划(2017YYSP005)
国家外国专家局2017年引进境外技术
管理人才项目计划(20173700017)
关键词
羊栖菜
硫酸化多糖
褐藻糖胶
抗氧化活性
Sargassum fusiforme
sulfated polysaccharide
fucoidan
antioxidant activities
