摘要
目的:优化白及花中脂溶性成分提取工艺,并初步探究其抑菌、抗肿瘤和抗氧化活性。方法:在单因素实验基础上,运用响应面法优化白及花中脂溶性成分提取工艺,以提高脂溶性成分得率,并通过滤纸片扩散法和CCK8法分别研究其抑菌和抗肿瘤活性,通过检测DPPH自由基、ABTS自由基和OH自由基的清除率考察其体外抗氧化活性。结果:最佳提取工艺如下:提取时间2.94 h,提取次数2次,料液比为1∶40时,此条件下脂溶性成分得率的理论值可达到1.416%,验证试验的平均得率为1.401%±0.112%,比优化前提高了28.49%。生物活性实验表明:白及花脂溶性成分对枯草芽孢杆菌(Bacillus subtilis)、金黄色葡萄球菌(Staphyloccocus aureus)、大肠埃希菌(Escherichia coli)、白色念珠菌(Candida albicans)四种致病菌有明显的抑菌效果;经CCK-8法测定,脂溶性成分对肺腺癌A549细胞的抑制率为32.115%;而抗氧化实验表明白及花中脂溶性成分对DPPH自由基、ABTS自由基和羟基自由基的EC50分别为1.63、0.47和1.25 mg/m L。结论:优化的提取工艺合理、可行,白及花脂溶性成分生物活性较好,具有潜在的药用价值。
Objective: To optimize the extraction procedure of liposoluble constituents in Bletilla striata flower by response surface methodology. And study on its bioactivity such as antibacterial activity,antitumor activity and antioxidant activity.Methods: Response surface methodology was used to design,optimize and verify the extraction procedure of liposoluble constituents in B.striata flower on the basis of single-factor experiments,so as to improve the yield of liposoluble component.It was evaluated for antibacterial activity by paper diffusion method,and antitumor activity by CCK8 method,while its antioxidant activity in vitro was evaluated by removal ability of DPPH·,ABTS+·and hydroxyl free radicals. Results: The results of optimum extraction condition were as follows: Extraction time 2.94 h,2 times of extraction,the material-liquid ratio 1∶ 40 g/m L. Under these conditions,the predicted values of the yield of liposoluble components could reach 1.416%,the average yield of the verification test was 1.401% ± 0.112%,which was 28.49% higher than before. Bioactivity tests showed that: Liposoluble components in B.striata flower had significantly inhibitory effect on Bacillus subtilis,Staphyloccocus aureus,Escherichia coli and Candida albicans four kind of pathogenic bacterias,as well as the inhibitory rate of A549 cells had raised 32.115% determinated by CCK-8 method.It also had inoxidizability and its EC50 was 1.63,0.47 and 1.25 mg/m L for DPPH free radical,ABTS free radical and hydroxyl radical respectively. Conclusion: The optimized extraction procedure was reasonable and feasible. The liposoluble components in B.striata flower had significant biological activity and potential medicinal value.
作者
解修超
刘军生
罗阳兰
邓百万
曹乃馨
贾娇
XIE Xiu-chao;LIU Jun-sheng;LUO Yang-lan;DENG Bai-wan;CAO Nai-xin;JIA Jiao(Shaanxi Provincial Engineering Research Center of Edible&Medicinal Fungi,Shaanxi University of Technology,Hanzhong 723001,China;School of Biological Science&Engineering,Shaanxi University of Technology,Hanzhong 723001,China)
出处
《食品工业科技》
CAS
北大核心
2019年第4期200-206,共7页
Science and Technology of Food Industry
基金
陕西省科技统筹创新工程计划项目(2016HBGC-07)
秦巴山区生物资源综合开发协同创新中心自然科学研究项目(QBXT-Z(P)-15-2
QBXT-17-12)
关键词
白及花
脂溶性成分
响应面分析
抗肿瘤活性
抗氧化活性
Bletilla striata
liposoluble components
response surface analysis
antitumor activity
antioxidant activity
