摘要
目的:确定高粱全粉中色素超声辅助提取最佳工艺条件,并对其抗氧化活性进行评价。方法:在单因素试验基础上,以色素提取率为响应值,应用响应面Box-Behnken试验设计对色素提取工艺条件进行优化;同时探究高粱色素的体外抗氧化能力。结果:超声波辅助提取高粱全粉色素的最佳条件为:乙醇体积分数50.76%、超声功率500.64 W、料液比1:16.72(g/mL),此条件下色素提取率理论值为54.26%,在提取条件为乙醇体积分数51%、超声功率为504 W、料液比为1∶17(g/mL)时,色素提取率为(53.99±0.26)%;高粱色素对1,1-二苯基苦基苯肼(1,1-diphenyl-2-picrylhydrazy,DPPH)自由基和羟自由基(·OH)清除作用明显,且具有较好的还原能力。结论:利用响应面Box-Behnken试验分析结果可靠,得到了高粱全粉色素超声波辅助提取的最佳工艺条件,实验结果表明高粱色素具有较强的抗氧化活性。
Objective: To explore the optimal ultrasonic-assisted extraction conditions of pigment from sorghum flour and evaluate the antioxidant activity of the extracted pigment. Methods: Based on the single-factor designs, the ultrasonicassisted extraction conditions of pigment from sorghum flour were optimized by response surface methodology with BoxBehnken design in order to increase the extraction yield of pigment. Meanwhile, the in vitro antioxidant activity of pigment from sorghum flour was also studied. Results: The optimal extraction conditions were 50.76%, 500.64 W and 1:16.72(g/mL) for ethanol concentration, ultrasonic power and material/liquid ratio, respectively. Under these conditions, the maximum predicted extraction yield of pigment was 54.26%. When the ethanol concentration, ultrasonic power and material/liquid ratio were 51%, 504 W and 1:17(g/mL), respectively, the experimentally observed extraction yield of pigment was(53.99 ± 0.26)%. Sorghum pigment showed significant scavenging effects on 1,1-diphenyl-2-picryl-hydrazy(DPPH·) and hydroxyl free radicals(·OH) and excellent reducing power. Conclusion: Response surface methodology with Box-Behnken design is applicable for the optimization of the ultrasonic-assisted extraction of pigment from sorghum flour. The pigment from sorghum flour has strong antioxidant capacity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第16期39-45,共7页
Food Science
基金
"十二五"农村领域国家科技计划课题(2012BAD34B05)
关键词
高粱色素
超声提取
响应面
抗氧化
sorghum pigment
ultrasonic-assisted extraction
response surface methodology
antioxidant activity
