摘要
目的:比较研究海地瓜岩藻聚糖硫酸酯(fucoidan from Acaudina molpadioidea,Am-FUC)和美国肉参岩藻聚糖硫酸酯(fucoidan from Isostichopus badionotus,Ib-FUC)对肥胖小鼠胰岛素抵抗及相关炎症因子的改善作用。方法:以高脂高果糖饲料饲喂法建立胰岛素抵抗小鼠模型。雄性C57BL/6小鼠随机分为正常对照组、模型对照组、阳性对照组、实验A、B、C、D组。正常对照组饲喂标准饲料,其他组饲喂高脂高果糖饲料。阳性对照组、实验A、B、C、D组分别在饲料中添加罗格列酮(rosiglitazone,RSG,1 mg/(kg·d))、低剂量Ib-FUC(20 mg/(kg·d))、高剂量Ib-FUC(80 mg/(kg·d))、低剂量Am-FUC(20 mg/(kg·d))、高剂量Am-FUC(80 mg/(kg·d))。各组小鼠自由摄食摄水120 d。实验结束后,称量小鼠白色脂肪质量,检测空腹血糖、血清胰岛素及血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、瘦素、脂联素水平。结果:2种FUC均可极显著地降低胰岛素抵抗小鼠的脂肪积累(P<0.01),降低血糖(P<0.01)和胰岛素(P<0.01)水平,改善胰岛素抵抗(P<0.01),降低TNF-α(P<0.01)、IL-6(P<0.01)和瘦素(P<0.01)含量,提高血清脂联素含量(P<0.01)。Am-FUC效果优于Ib-FUC。结论:Am-FUC和Ib-FUC能显著改善胰岛素抵抗小鼠的高血糖症状及胰岛素抵抗程度,Am-FUC的效果优于Ib-FUC,其作用机制可能与改善肥胖引起的细胞因子的分泌有关。
The aim of the present study was to unravel the effects of fucoidan from Acaudina molpadioidea(Am-CHS) versus Isostichopus badionotus(Ib-CHS) on insulin resistance improvement and related inflammatory cytokines in obese mice. Male C57BL/6 mice were randomly divided into normal control, model control, positive control, and experimental groups A, B, C and D. The mice in normal control group were fed standard diet, and those in other groups were fed high-fat, high-fructose diet. Rosiglitazone(RSG, 1 mg/(kg·d)), low-dose Ib-FUC(20 mg/(kg·d)), high-dose Ib-FUC(80 mg/(kg·d)), low-dose Am-FUC(20 mg/(kg·d)) and high-dose Am-FUC(80 mg/(kg·d)) were added into the diets of positive control group, and experimental groups A, B, C and D, respectively. All animals were fed with diets and allowed to drink water freely for 90 days. After the experiments, white fat weight, fasting serum glucose, serum insulin, serum tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), leptin, and adiponectin levels were determined. Results showed that Am-CHS and Ib-FUC could significantly decrease fat deposition(P < 0.01), blood glucose(P < 0.01) and insulin(P < 0.01) levels, ameliorate insulin resistance, increase serum adiponectin concentration, lower serum TNF-α(P < 0.01), IL-6(P < 0.01) and leptin(P < 0.01) levels, and enhance adiponectin levels(P < 0.01). The effects of Am-FUC were more significant than those of Ib-FUC(P < 0.01). Both these compounds significantly decreased blood glucose level and ameliorated insulin resistance in insulin-resistant mice, and the mechanisms might involve the regulation of adipocytokines parasecretion caused by adiposity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第21期201-206,共6页
Food Science
基金
海洋公益性行业科研专项(201105029)
山东省自主创新专项(2012CX80201)
关键词
海地瓜
美国肉参
岩藻聚糖硫酸酯
胰岛素抵抗
炎症因子
Acaudina molpadioidea
Isostichopus badionotus
fucoidan
insulin resistance
inflammatory cytokine
