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嗜酸乳杆菌细菌素Lactobacillin XH2抑制大肠杆菌作用机理的探讨 被引量:17

Antibacterial Mechanism of Lactobacillin XH2 from Lactobacillus acidophilus on Escherichia coli
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摘要 将离子交换色谱纯化获得的抑菌效价为380.60 AU/mL的嗜酸乳杆菌细菌素Lactobacillin XH2作用于大肠杆菌(Escherichia coli),从死亡率、细胞膜损伤及引起胞内物质外泄方面,探讨Lactobacillin XH2抑制大肠杆菌的机理。结果表明:Lactobacillin XH2作用于大肠杆菌后,在3 h内大肠杆菌死亡率达96%,菌体细胞变形并出现孔洞,菌体短小且不规整。进一步分析发现,细胞膜通透性增大,胞内K+快速大量泄露,膜电位紊乱失衡;胞内紫外吸收物质、乳酸脱氢酶等生命大分子物质大量流失;同时导致了大肠杆菌胞内三磷酸腺苷(adenosine triphosphate,ATP)外泄,细胞物质代谢、能量代谢失常,最终导致了菌体死亡。这些现象表明,孔道形成(pore formation)理论可能同样适用于Lactobacillin XH2对革兰氏阴性菌大肠杆菌的作用机理。 Lactobacillin XH2 with antibacterial potency of 380.60 AU/mL was purified from the culture supernatant of Lactobacillus acidophilus by ion-exchange chromatography. Its antibacterial mechanism on Escherichia coli (E. coli) was investigated by examining its effects on mortality rate, cell membrane damage and leakage of intracellular contents from E. coli cells. Most E. coli cells died in 3 h and their cell walls were partially damaged. The concentration of intracellular K+ in E. coli decreased rapidly, which resulted in imbalance of the membrane potential. The leakage of large amounts of macromolecules such as ultraviolet-absorbing substances, lactate dehydrogenase and intracellular ATP led to disruption of the cellular structure and abnormal energy metabolism, causing the cells to die eventually. These observations i ndicate that the pore formation theory could apply to Gram-negative bacteria E. coli.
出处 《食品科学》 EI CAS CSCD 北大核心 2015年第3期75-79,共5页 Food Science
基金 河南省教育厅科学技术研究重点项目(12A550004) 河南省科技攻关计划项目(122102110117) 新乡市科技创新平台建设项目(CP1310)
关键词 嗜酸乳杆菌 细菌素 革兰氏阴性菌 抑菌机理 Lactobacillus acidophilusbacteriocin Gram-negative bacteria antibacterial mechanism
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