摘要
目的:探讨硫酸氨基葡萄糖(glucosamine sulfate,GS)对口服透明质酸(hyaluronic acid,HA)缓解小鼠佐剂性关节炎的增效作用。方法:将50只8周龄ICR雄性小鼠随机分成5组:正常组、模型组、HA组、GS组和HA+GS组。除正常组以外,其余4组小鼠左后足注射弗氏完全佐剂(freund’s complete adjuvant,FCA)建立佐剂性关节炎模型,然后进行4周的HA和GS口服给药,并分别对小鼠体质量、足跖肿胀度进行测定,同时采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测小鼠血清中白细胞介素-1(interleukin-1,IL-1)及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的含量。结果:口服HA和GS都能够减轻小鼠的足跖肿胀度,而同时口服HA和GS能起到更好的消肿效果,血清中IL-1及TNF-α的含量也显著降低。结论:给予小鼠口服HA+GS,比单独服用HA、GS能更有效、更快地缓解小鼠的足跖肿胀症状,同时能够降低血清中IL-1及TNF-α含量,进而对小鼠佐剂性关节炎起到更好的治愈效果。
Aim:To explore the synergism of glucosamine sulfate(GS) with hyaluronic acid(HA) by oral administration in relieving adjuvant arthritis in mice with.Methods:Fifty 8-week-old ICR male mice were randomly divided into 5 groups at random with an equal number of animals in each group:normal,model,HA,GS,and HA + GS groups.Except normal group,the animal models with adjuvant arthritis from 4 other groups were established by injecting 0.1 m L of Freund's complete adjuvant(FCA) into the left rear paw,followed by oral administration of HA and/or GS for 4 weeks.The body weights and swelling degree of mouse rear feet were evaluated respectively.The serum concentration of IL-1 and TNF-α in mice were detected by enzymelinked immunosorbent assay(ELISA).Results:Swelling degree of mouse feet could be inhibited by oral administration of HA or GS,and this inhibitory effect was more pronounced when both compounds were administrated.In addition,an obvious decrease in IL-1 and TNF-α levels in serum was observed.Conclusion:In addition to decreasing serum IL-1 and TNF-α levels,HA and GS could alleviate the swelling of mouse feet more effectively and rapidly when used in combination than when used individually,so that exerting a better curative effect on mouse arthritis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第5期189-194,共6页
Food Science
基金
上海海洋大学-日本丘比株式会社合作研究基金项目
关键词
口服透明质酸
口服硫酸氨基葡萄糖
佐剂性关节炎
oral administration of hyaluronic acid
oral administration of glucosamine sulfate
adjuvant arthritis
