摘要
以菌群人源化(human flora-associated,HFA)小鼠为研究模型,探讨燕麦β-葡聚糖(oatβ-glucan,OG)与沙蒿胶多糖(Artemisia sphaerocephala Krasch.polysaccharide,ASP)对HFA小鼠生理及肠道微生物的不同影响。通过接种健康人志愿者的粪便悬液构建HFA小鼠模型,将30只HFA小鼠随机分为普通组(CT组)、燕麦β-葡聚糖组(CT+OG组)和沙蒿胶多糖组(CT+ASP组),分别用基础饲料和添加质量分数5%燕麦β-葡聚糖或5%沙蒿胶多糖饲料饲喂HFA小鼠8周,进行血清生化指标测定、肝脏和脂肪苏木精-伊红(hematoxylin-eosin,HE)染色、肠道菌群聚合酶链式反应-变性梯度凝胶电泳(polymerase chain reaction-denaturing gradient gel electrophoresis,PCR-DGGE)检测。结果表明:与CT组比较,两种多糖均能降低HFA小鼠空腹血糖水平(P>0.05),CT+OG组能显著降低甘油三酯含量(P<0.05),CT+ASP组总胆固醇含量和高密度脂蛋白胆固醇含量显著高于其他两组(P<0.05);CT+OG组脂肪细胞较CT+ASP组、CT组明显变小,细胞排列更加紧密;多糖饮食会增加HFA小鼠肠道的微生物多样性,CT+ASP组效果优于CT+OG组(P>0.05),说明多糖饮食会明显改变HFA小鼠生理及肠道菌群结构,而且不同多糖饮食对于HFA小鼠的肠道菌群组成和丰富度的作用差异与多糖分子质量大小有关。在降低HFA小鼠血糖血脂含量、脂肪细胞大小方面,分子质量小的燕麦β-葡聚糖功效明显优于分子质量大的沙蒿胶多糖;但在增加HFA模型小鼠肠道微生物多样性方面,分子质量大的沙蒿胶多糖优于分子质量小的燕麦β-葡聚糖。
In order to compare the effects of oat beta-glucan and Artemisia sphaerocephala Krasch. polysaccharide(ASP) on the physiological parameters and the structure of the gut microbiota, human flora-associated(HFA) mouse model was established by inoculating 30 germ-free mice with fecal suspension derived from a healthy volunteer. The HFA mice were randomly divided into three groups: the first group was fed with normal diet(CT group), the second group was fed with normal diet containing 5% oat beta-glucan(CT + OG group), and the third group was fed with normal diet with 5% ASP(CT + ASP group). After 8 weeks of feeding, serum biochemical indexes were determined, pathological sections of liver were stained with HE and fat levels were evaluated, and the change in the gut microbiota was analyzed by PCR-DGGE(denaturing gradient gel electrophoresis). The results showed that the two kinds of polysaccharides reduced fasting serum glucose level in HFA mice(P > 0.05) when compared with CT group, CT + OG group significantly reduced the content of total triglyceride(P < 0.05), and total cholesterol content and high density lipoprotein cholesterol content in CT+ASP group were significantly higher than those of CT and CT + OG group(P < 0.05). CT + OG group significantly decreased the volume of fat cells obviously and displayed more dense arrangement of fat cells when compared CT + ASP group and CT group. Moreover, the structure of the gut microbiota in polysaccharide-diet-fed HFA mice changed; their gut bacterial diversity index increased, and the effect of ASP was superior to that of oat beta-glucan(P > 0.05). Polysaccharide diet obviously altered physiological parameters and structure of gut microbiota in HFA mice. The composition and bacteria abundance of intestinal flora in different polysaccharide-diet-fed HFA mice exhibited different changes which were associated with the molecular weight of polysaccharides. In term of reducing the levels of fasting serum glucose, serum total cholesterol and triglyceride, and the size of fat cells, the effect of low molecular weight oat beta-glucan was obviously better than that of high molecular weight ASP in HFA mice. When it comes to increasing intestinal microbial diversity, the effect of ASP was superior to oat beta-glucan distinctly.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第9期146-153,共8页
Food Science
基金
国家自然科学基金面上项目(31371840)
国家现代农业(燕麦荞麦)产业技术体系建设专项(CARS-08-D1)
关键词
菌群人源化小鼠
燕麦Β-葡聚糖
沙蒿胶多糖
聚合酶链式反应-变性梯度凝胶电泳
human flora-associated mice
oat beta-glucan
Artemisia sphaerocephala Krasch.polysaccharide
polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE)
