摘要
用胶体金和纳米金-聚苯胺-纳米金微球(GPG)分别标记制备的沙丁胺醇多克隆抗体,喷涂在玻璃纤维垫上,将包被抗原和羊抗兔二抗分别固定到硝酸纤维素膜上作为检测线(T-Line)和质控线(C-Line),干燥后组装试纸条,建立免疫层析分析方法。考查检测试纸条的灵敏度和稳定性,并应用于动物源性食品(猪肉、牛肉、羊肉和猪肝)中沙丁胺醇残留的可视化检测。实验结果可以看出,沙丁胺醇胶体金标记免疫层析分析方法的样品检出限为5μg/L,沙丁胺醇GPG标记免疫层析分析方法的样品检出限为10μg/L。
Colloidal gold and nanogold-polyaniline-nanogold microspheres (GPGs) were labeled purified salbutamol polyclonal antibodies, respectively. And then they were spraide on glass fiber mat, the coating anti-gen and goat anti-rabbit secondary antibody were fixed to nitrocellulose membrane as test line and control line respectively.After drying assembled test strip, the immunochromatographic strip for the determination of salbu-tamol were deceloped. The sensitivity and stability of the test strip were studied and it was applied in the visual detection of salbutamol residued in animal food (pork, beef, lamb and liver). It was found that the detection limit of colloidal gold immunochromatographic test strip were 5μg/L in samples,while the detection limit of im-munochromatographic rapid test strip labeled by GPG were 10μg/L.
出处
《食品研究与开发》
CAS
北大核心
2016年第18期124-128,共5页
Food Research and Development
基金
质检公益性行业科研专项(201310146)
