摘要
以猪特异性基因序列为靶位点设计特异性引物,以常见畜禽肉包括猪肉、羊肉、兔肉、牛肉、鸽肉、鹌鹑肉、鸡肉、鸭肉、鹅肉等参考动物肌肉DNA为模板,进行荧光定量PCR扩增,建立猪源性成分荧光定量PCR检测方法;并将猪肉DNA模板浓度进行8个梯度稀释,检测其灵敏度。结果显示,该方法能够有效对猪源性成分进行快速检测,具有较强的特异性,灵敏度较高,可快速准确地鉴别畜禽肉中猪源性成分。
In order to establish a fluorogenic quantitative PCR method,which could identify the components of pig origin,specific genes sequences of pig was used as target sites,and the specific primers were designed. The DNA of common livestock and poultry meat including mutton,pork,rabbit meat,beef,pigeon meat,quail meat,chicken,duck and goose were used as template. And the template was diluted in eight gradients,for detecting the sensitivity. The results showed that the established method could detect the pig origin effectively,which had a quite specificity and high sensitivity. The method could detect the pig origin in livestock and poultry meat quickly and accurately.
出处
《食品研究与开发》
CAS
北大核心
2017年第10期110-112,172,共4页
Food Research and Development
基金
扬州市社会发展前瞻性研究项目(YZ2014188)
扬州市科技公共服务平台建设(YZ2015162)
2016国家农产品质量安全风险评估项目(GJFP2016007)
关键词
动物源性成分
荧光定量PCR
特异性基因
检测
animal origin ingredients
fluorogenic quantitative PCR
specific genes
identification
