摘要
紫薯和红曲均被认为具有保护肝脏的功能,但紫薯经红曲发酵后护肝作用的变化未见报道。为研究红曲发酵对紫薯干预肝细胞糖脂代谢能力的影响,首先检测紫薯发酵前后成分变化,并采用油酸联合果糖诱导Hep G2细胞建立糖脂代谢紊乱模型,测定了紫薯和红曲紫薯对模型细胞葡萄糖消耗量和糖原、甘油三酯(TG)、总胆固醇(TC)含量的影响,并测定了细胞内活性氧自由基(ROS)的水平。结果表明,经红曲发酵后,紫薯的总酚含量及Fe^(3+)还原能力上升,总黄酮含量下降。紫薯和红曲紫薯均可显著增加模型细胞的葡萄糖消耗量和糖原含量(P<0.05),抑制TG和TC的合成,降低ROS水平。发酵后紫薯促进模型细胞糖原合成能力约提升60%,下调TC能力约提升1倍,说明紫薯经过红曲菌发酵后,其干预HepG2糖脂代谢紊乱细胞糖脂代谢能力增强,可能与紫薯发酵后多酚含量提升有关。
Both purple sweet potato(PSP) and red yeast rice are believed to have the function of protecting the liver, but the function of red yeast fermented PSP(FPSP) has not been reported. In order to investigate the effect of red yeast fermentation on the ability of PSP to regulate the cell glycolipid metabolism, the components of PSP before and after fermentation were firstly tested. The cell model of glycolipid metabolism disorder was induced by oleic acid combined with fructose. The glucose consumption, the glycogen, triglyceride(TG) or total cholesterol(TC) content and the level of reactive oxygen species(ROS) of model cells were tested to determine the effects of PSP and FPSP. The results showed that the total phenolic content and the reducing ability of Fe3+ increased and the total flavonoids decreased after the fermentation. Both PSP and FPSP could increase the glucose consumption and glycogen content of the model cells significantly(P<0.05) and inhibit the synthesis of TG and TC. They could aslo decrease the ROS levels. After fermentation, the ability of PSP to promote the synthesis of glycogen in the model cell increased by about 60%, and the ability to down-regulate the TC increased by about one-fold, indicating that after fermentation the ability of regulating glycolipids metabolism was enhanced. The reason for this result may be associate with the increased polyphenol content of the PSP after fermentation.
作者
周文萱
柳嘉
潘聪
田巧基
刘义凤
李耀
赵吉兴
段盛林
ZHOU Wenxuan;LIU Jia;PAN Cong;TIAN Qiaoji;LIU Yifeng;LI Yao;ZHAO Jixing;DUAN Shenglin(China Food Fermentation Research Institute Co.,Ltd.,Beijing Key Laboratory of the Innovative Development of Functional Staple and the Nutritional Intervention for Chronic Disease,Beijing 100015;Jilin Agricultural University,Changchun 130118;Shandong Zhonghui Biotechnology Co.,Ltd.,Huimin 251706)
出处
《食品科技》
CAS
北大核心
2019年第1期241-247,共7页
Food Science and Technology
基金
北京市科技计划项目(D171100001917003)
关键词
红曲
紫薯
肝癌细胞HEPG2
糖脂代谢
Monascus ruber
purple sweet potato
HepG2
glycolipids metabolism
